Bioorthogonal reactions challenged: DNA templated native chemical ligation during PCR
Bioorthogonal reactions challenged: DNA templated native chemical ligation during PCR
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DOI：
10.1039/c2sc20961f
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发表时间：
2013-01-01
期刊：
影响因子：
8.4
通讯作者：
Seitz, Oliver
中科院分区：
文献类型：
--
作者：
Roloff, Alexander;Seitz, Oliver
DNA定向化学通常通过使用纳摩尔量的DNA模板进行。在这里，我们介绍了一种方法，允许使用阿托摩尔模板负载。DNA模板化的天然化学连接产生两个荧光团的共价固定，同时在聚合酶链式反应期间产生模板。
DNA directed chemistry is commonly performed by using nanomolar amounts of DNA templates. Herein we introduce a method that allows the use of attomolar template loads. A DNA templated native chemical ligation yields to the covalent fixation of two fluorophores while the template is being produced during polymerase chain reaction.