Opsonophagocytic activity and other serological indications of Bordetella pertussis infection in military recruits in Norway
Opsonophagocytic activity and other serological indications of Bordetella pertussis infection in military recruits in Norway
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DOI：
10.1128/cvi.00081-07
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发表时间：
2007-07-01
影响因子：
--
通讯作者：
Aaberge, Ingeborg S.
中科院分区：
文献类型：
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作者：
Aase, Audun;Herstad, Tove Karin;Aaberge, Ingeborg S.
百日咳博德特氏菌是百日咳（百日咳）的病原体。尽管疫苗接种覆盖率很高，但百日咳在许多国家仍然是一种重要疾病。除了疫苗接种外，博德特氏菌属的短暂携带。或其他交叉反应（微生物）增加了针对百日咳的免疫力。然而，提供保护的各种免疫机制仍然很大程度上未知。在这项研究中，收集了 464 名健康挪威新兵的配对血清样本，第一次是在入伍时收集的，第二次是在大约 8 个月后收集的。通过酶联免疫吸附测定比较配对血清样本中针对百日咳毒素 (PT) 的免疫球蛋白 G (IgG) 抗体，检查了服兵役期间百日咳的患病率(ELISA) 78% 的新兵在两个样本中都具有低水平的 PT IgG 抗体，相反，8.4% 的新兵在第一个样本中表现出高抗 PT IgG 水平，表明一名新兵在入伍前经历了血清转化，这表明通过不同的 ELISA 试剂盒对 248 份具有低、中和高抗 PT IgG 滴度的血清样本进行了分析。针对 PT 和丝状血凝素 (FHA) 的 IgG 和 IgA 抗体、调理吞噬活性 (OPA)、诱导 C3b 沉积产物以及与活百日咳博德特氏菌作为抗原结合的 IgG 我们观察到针对活细菌的 OPA 和 IgG 之间 (r = 0.83)、OPA 和抗 FHA IgG 之间 (r = 0.79)、OPA 和抗 PT IgG 之间 (r = 0.79) 之间存在高度相关性。 0.68），以及 OPA 和 C3b 结合之间的关系（r = 0.70）（所有 P < 0.0001）与其他检测没有密切相关。
Bordetella pertussis is the causative agent of pertussis (whooping cough). Despite high vaccination coverage, pertussis remains a significant disease in many countries. Besides vaccination, transient carriage of Bordetella spp. or other cross-reacting (organisms adds to the immunity against pertussis. However, the various immunological mechanisms conferring protection remain largely unknown. In this study, paired serum samples from 464 healthy Norwegian military recruits were collected, the first at enrolment and the second about 8 months later. The prevalence of pertussis during military service was examined by comparing the paired serum samples for immunoglobulin G (IgG) antibodies against pertussis toxin (PT) by enzyme-linked immunosorbent assay (ELISA). Seventy-eight percent of the recruits had low levels of IgG antibodies against PT in both samples. Conversely, 8.4% of the recruits demonstrated high anti-PT IgG levels in the first sample, indicative of recent pertussis prior to enrolment. One recruit experienced seroconversion, indicating pertussis during service. A subset of 248 serum samples with low, medium, and high anti-PT IgG titers were analyzed by a different ELISA kit for IgG and IgA antibodies against PT and filamentous hemagglutinin (FHA) and for opsonophagocytic activity (OPA), for induction of C3b deposition products, and for IgG binding with live B. pertussis as the antigen. We observed high correlations between OPA and IgG against live bacteria (r = 0.83), between OPA and IgG anti-FHA (r = 0.79), between OPA and anti-PT IgG (r = 0.68), and between OPA and C3b binding (r = 0.70) (P < 0.0001 for all). Anti-PT IgA did not correlate closely with the other assays.