The beginning of pattern formation in the Drosophila compound eye: the morphogenetic furrow and the second mitotic wave.
The beginning of pattern formation in the Drosophila compound eye: the morphogenetic furrow and the second mitotic wave.
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作者：
T. Wolff;D. Ready
在果蝇中，形态发生沟中的事件为所有随后的复眼发育奠定了基础。成年人眼睛的周期性模式开始于沟与间隔的小眼雏形，前集群的起始。有丝分裂的波紧跟着皱纹。一个细胞一个细胞的分析揭示了这些事件的细节。小眼组装的早期阶段可以用硫化铅染色来解决。当细胞聚集成周期性间隔的同心聚集体时，明显的小眼组织开始于形态发生沟。一个固定的细胞重排序列将这些聚集体转化为前簇。在沟中，新的小眼行开始在赤道和增长作为新的集群添加到周边端。使用BrdU饲料的有丝分裂标记显示，所有的细胞不纳入一个precluster分裂。BrdU注射显示细胞在两个相邻的小眼行之间大致同时分裂。
Events in the morphogenetic furrow set the stage for all subsequent compound eye development in Drosophila. The periodic pattern of the adult eye begins in the furrow with the spaced initiation of ommatidial rudiments, the preclusters. A wave of mitosis closely follows the furrow. A cell-by-cell analysis reveals details of these events. Early stages of ommatidial assembly can be resolved using a lead sulfide stain. Overt ommatidial organization begins in the morphogenetic furrow as cells gather into periodically spaced concentric aggregates. A stereotyped sequence of cell rearrangements converts these aggregates into preclusters. In the furrow, new rows of ommatidia are initiated at the equator and grow as new clusters are added to the peripheral ends. Mitotic labeling using BrdU feeds shows that all cells not incorporated into a precluster divide. BrdU injections show that cells divide roughly simultaneously between two adjacent rows of ommatidia.