Restoration of gap junctional intercellular communication by caffeic acid phenethyl ester (CAPE) in a ras-transformed rat liver epithelial cell line
Restoration of gap junctional intercellular communication by caffeic acid phenethyl ester (CAPE) in a ras-transformed rat liver epithelial cell line
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DOI：
10.1016/s0304-3835(00)00470-5
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发表时间：
2000-08-31
期刊：
影响因子：
9.7
通讯作者：
Trosko, JE
中科院分区：
文献类型：
--
作者：
Na, HK;Wilson, MR;Trosko, JE
Caffeic acid phenethyl ester (CAPE), an active ingredient of honeybee propolis, has been identified as having anti-inflammatory, anti-viral and anti-cancer properties. Since the deficiency of gap junctional intercellular communication (GJIC) has been shown to be a characteristic of most cancer cells, this study was designed to test the hypothesis that the anti-carcinogenic activity of CAFE might be related to its ability to restore GJIC in tumorigenic GJIC-deficient cells (WB-ras2 cells). The results showed that CAFE restored GJIC, phosphorylation of connexin 43 (Cx43) and its normal localization on the plasma membrane in WB-ras2 cells after 3 days at 5 mug/ml concentration. Additionally, CAFE inhibited growth in soft agar and decreased the protein level of p21(ras). The results are consistent with the hypothesis that the anti-cancer mechanism of CAFE may be mediated by its ability to restore GJIC. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.
