Construction of antibacterial multilayer films containing nanosilver via layer-by-layer assembly of heparin and chitosan-silver ions complex
Construction of antibacterial multilayer films containing nanosilver via layer-by-layer assembly of heparin and chitosan-silver ions complex
复制标题
DOI：
10.1002/jbm.a.30819
复制
发表时间：
2006-12-01
影响因子：
4.9
通讯作者：
Shen, Jiacong
中科院分区：
文献类型：
--
作者：
Fu, Jinhong;Ji, Jian;Shen, Jiacong
采用逐层方式制备了含有纳米银的抗菌多层膜。采用1，6-己二胺胺解PET膜，在膜表面引入氨基，壳聚糖-硝酸银配合物和肝素交替沉积在膜表面，抗坏血酸还原膜内的银离子，形成银纳米粒子。紫外-可见光谱和透射电子显微镜证实形成了良好分散的纳米银粒子的大小（10-40 nm），这取决于壳聚糖溶液中的初始浓度的银离子和抗坏血酸溶液的pH值。壳聚糖/肝素多层膜对大肠杆菌（Escherichia coli，E. coli），以及将银纳米颗粒掺入到多层膜中。含纳米银的多层膜不仅是有效的抗菌，但也作为抗凝血涂层。细胞毒性评价表明，含纳米银的多层膜没有表现出任何细胞毒性。含纳米银的多层膜在医疗器械，特别是心血管植入物的表面改性方面具有良好的应用前景。(c)2006 Wiley Periodicals，Inc.
Antibacterial multilayer films containing nanosilver were prepared via layer-by-layer fashion. PET film was aminolyzed with 1,6-hexanediamine to introduce amino groups on PET film surface; chitosan-silver nitrate complex and heparin were alternately deposited onto an aminolyzed PET film surface, and subsequently, the silver ions within the multilayer films were reduced with ascorbic acid to form silver nanoparticles. UV-visible spectroscopy and transmission electron microscopy confirmed the formation of well-dispersed nanosilver particles with sizes (10-40 nm) that depended on the initial concentration of silver ions in chitosan solution and the pH of ascorbic acid solution. The chitosan/heparin multilayer films were possessed of bactericidal effect on Escherichia coli (E. coli), and the incorporation of silver nanoparticles into the multilayer films. The multilayer films containing nanosilver were not only effective as antibacterial but also as anticoagulant coating. And cell toxicity evaluation suggested that the multilayer films containing nanosilver did not show any cytotoxicity. The multilayer films containing nanosilver may have good potentials for surface modification of medical devices, especially for cardiovascular implants. (c) 2006 Wiley Periodicals, Inc.