Overexpression of the Transcription Factors GmSHN1 and GmSHN9 Differentially Regulates Wax and Cutin Biosynthesis, Alters Cuticle Properties, and Changes Leaf Phenotypes in Arabidopsis.
Overexpression of the Transcription Factors GmSHN1 and GmSHN9 Differentially Regulates Wax and Cutin Biosynthesis, Alters Cuticle Properties, and Changes Leaf Phenotypes in Arabidopsis.
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拟南芥中转录因子 GmSHN1 和 GmSHN9 的过度表达差异调节蜡和角质生物合成、改变角质层特性并改变叶子表型
DOI：
10.3390/ijms17040587
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发表时间：
2016-04-21
影响因子：
5.6
通讯作者：
Gu D
中科院分区：
文献类型：
--
作者：
Xu Y;Wu H;Zhao M;Wu W;Xu Y;Gu D
SHINE（SHN/WIN）进化枝蛋白是植物APETALA 2/乙烯反应元件结合因子（AP 2/ERF）家族的转录因子，已被证实参与蜡质和角质的生物合成。大豆是一种重要的经济作物，但其蜡质生物合成的分子机制研究较少。本研究从大豆中鉴定了10个拟南芥SHN基因的同源物。这些同源物在基因结构和器官表达模式上存在差异。每个大豆SHN基因在拟南芥中的组成型表达导致不同的叶片表型，以及不同水平的叶片表面光泽度。GmSHN 1和GmSHN 9在拟南芥中的过表达分别表现出7.8倍和9.9倍的上调叶角质层蜡的产生。C31和C29烷烃对蜡含量的增加贡献最大。在GmSHN 1和GmSHN 9过表达的植株中，叶片总角质含量分别增加了11.4倍和5.7倍，主要是通过增加C16：0二羟基和二元酸。GmSHN 1和GmSHN 9还改变了转基因拟南芥叶片角质层膜的超微结构，增加了水分的流失速率。在GmSHN 1和GmSHN 9过表达中，许多蜡质和角质生物合成以及叶片发育相关基因的转录水平发生了改变。总之，这些结果表明，GmSHN 1和GmSHN 9可能差异调节叶发育过程以及蜡和角质的生物合成。
SHINE (SHN/WIN) clade proteins, transcription factors of the plant-specific APETALA 2/ethylene-responsive element binding factor (AP2/ERF) family, have been proven to be involved in wax and cutin biosynthesis. Glycine max is an important economic crop, but its molecular mechanism of wax biosynthesis is rarely characterized. In this study, 10 homologs of Arabidopsis SHN genes were identified from soybean. These homologs were different in gene structures and organ expression patterns. Constitutive expression of each of the soybean SHN genes in Arabidopsis led to different leaf phenotypes, as well as different levels of glossiness on leaf surfaces. Overexpression of GmSHN1 and GmSHN9 in Arabidopsis exhibited 7.8-fold and 9.9-fold up-regulation of leaf cuticle wax productions, respectively. C31 and C29 alkanes contributed most to the increased wax contents. Total cutin contents of leaves were increased 11.4-fold in GmSHN1 overexpressors and 5.7-fold in GmSHN9 overexpressors, mainly through increasing C16:0 di-OH and dioic acids. GmSHN1 and GmSHN9 also altered leaf cuticle membrane ultrastructure and increased water loss rate in transgenic Arabidopsis plants. Transcript levels of many wax and cutin biosynthesis and leaf development related genes were altered in GmSHN1 and GmSHN9 overexpressors. Overall, these results suggest that GmSHN1 and GmSHN9 may differentially regulate the leaf development process as well as wax and cutin biosynthesis.