The fluffy gene of Neurospora crassa encodes a Gal4p-type C6 zinc cluster protein required for conidial development.
The fluffy gene of Neurospora crassa encodes a Gal4p-type C6 zinc cluster protein required for conidial development.
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粗糙脉孢菌的蓬松基因编码分生孢子发育所需的 Gal4p 型 C6 锌簇蛋白。
DOI：
10.1093/genetics/148.4.1813
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发表时间：
1998
期刊：
影响因子：
3.3
通讯作者：
Ebbole,DJ
中科院分区：
文献类型：
--
作者：
Bailey,LA;Ebbole,DJ
粗糙脉孢菌蓬松突变体不能产生大分生孢子。我们克隆了fl基因以确定其在分生孢子形成调控中的作用。通过互补鉴定了含有fl的粘粒克隆。fl基因编码一个Gal 4p型C6锌簇蛋白，与N. crassa NIT 4蛋白，调节硝酸盐利用所需的基因。几个fl突变体等位基因的分析表明，无效突变体被阻止在萌芽阶段的发展需要产生分生孢子梗。fl mRNA是短暂诱导的发育承诺之前，萌芽生长。FL表达的时间与FL蛋白在先前表征的分生孢子特异性（con）基因con-6和con-10的激活中的作用一致。这些数据表明，FL作为一个发育调控的转录因子所需的分生孢子形态发生。
Neurospora crassa fluffy (fl) mutants are unable to produce macroconidia. We cloned the fl gene to determine its role in regulating conidiation. A cosmid clone containing fl was identified by complementation. The sequence of fl revealed that it encodes a Gal4p-type C6 zinc cluster protein with greatest similarity to the N. crassa NIT4 protein that regulates genes required for nitrate utilization. Analysis of several fl mutant alleles demonstrated that null mutants are blocked in the budding phase of development required to produce conidiophores. fl mRNA is transiently induced just prior to the developmental commitment to budding growth. This timing of fl expression is consistent with a role for FL protein in activation of the previously characterized conidiation-specific (con) genes, con-6 and con-10. These data suggest that FL acts as a developmentally regulated transcription factor required for conidiophore morphogenesis.