Discrepancies between morphologic, cytochemical, and immunologic characteristics in acute myeloblastic leukemia.
Discrepancies between morphologic, cytochemical, and immunologic characteristics in acute myeloblastic leukemia.
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急性髓细胞白血病形态学、细胞化学和免疫学特征之间的差异。
DOI：
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发表时间：
1987
影响因子：
3.5
通讯作者：
A. López‐borrasca
中科院分区：
文献类型：
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作者：
M. D. del Cañizo;J. S. San Miguel;M. González;J. Anta;A. Órfão;A. López‐borrasca
本研究旨在比较108例根据法国-美国-英国（FAB）标准分类的急性髓母细胞白血病（AML）的细胞化学模式和免疫表型。特别注意的是，这些方法之间存在差异的病例，以及一组11例被认为无法分类的患者，主要是因为检测到第二种细胞群——巨核母细胞。观察到三种类型的差异：具有典型的形态特征和细胞化学，但缺乏谱系特异性抗原的病例；这些主要包括低分化白血病（8个M1, 4个M2和8个M5a），这表明细胞化学酶是比目前可用的单克隆抗体更早的髓细胞标志物；免疫特征与形态学特征和细胞化学不一致的病例；其中包括2例单克隆抗体（CD14）阳性M2病例；6例M5b粒细胞单克隆抗体（CD15）阳性；缺乏CD14或CD15抗原的M4病例7例；形态学特征与细胞化学差异的病例，其中免疫标记允许正确评估细胞谱系（6例）。这些结果表明，结合形态学，细胞化学和免疫学方法可以更好地实现这些患者的分类。
This study was designed to compare the cytochemical pattern with the immunologic phenotype in 108 cases of acute myeloblastic leukemia (AML) classified according to the French-American-British (FAB) criteria. Special attention was paid to the cases where discrepancy existed between these approaches and to a group of 11 patients considered as unclassifiable mainly because a second cell population--megakaryoblastic--was detected. Three types of discrepancies were observed: cases with typical morphologic characteristics and cytochemistry but devoid of lineage-specific antigens; these mainly include poorly differentiated leukemias (eight M1, four M2, and eight M5a), suggesting that the cytochemical enzymes are earlier myeloid markers than the currently available monoclonal antibodies; cases in which immunologic characteristics were discordant with morphologic characteristics and cytochemistry; these include two M2 cases positive for monocytic monoclonal antibodies (CD14); six M5b cases positive for granulocytic monoclonal antibodies (CD15); and seven M4 cases lacking in CD14 or CD15 antigens; cases with discrepancies between morphologic characteristics and cytochemistry and in which the immunologic markers permitted the correct assessment of cell lineage (six cases). These results show that the classification of these patients is better achieved by a combined morphologic, cytochemical, and immunologic approach.