rpoD gene expression as an indicator of bacterial pathogens in host plants
rpoD gene expression as an indicator of bacterial pathogens in host plants
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DOI：
10.1007/s10327-011-0298-x
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发表时间：
2011-03-01
影响因子：
1.2
通讯作者：
Narusaka, Yoshihiro
中科院分区：
文献类型：
--
作者：
Narusaka, Mari;Shiraishi, Tomonori;Narusaka, Yoshihiro
假单胞菌属、拉尔斯顿氏菌属和黄单胞菌属是影响商业栽培作物的重要植物病原菌属。我们开发了一种方法，通过使用定量逆转录聚合酶链式反应 (qRT-PCR) 检测 rpoD mRNA，评估拟南芥中细菌病原体的生存力和发育。 rpoD 是一个代表性的管家基因，编码主要的 RNA 聚合酶 sigma 因子。通过 qRT-PCR 对 rpoD mRNA 进行定量可以轻松检测受感染植物组织中病原体的活力和发育情况。因此，qRT-PCR技术为研究宿主-病原体相互作用提供了新的研究机会。
Pseudomonas, Ralstonia, and Xanthomonas are important genera of plant pathogenic bacteria that affect commercially cultivated crops. We developed an assay to assess the viability and development of bacterial pathogens in Arabidopsis thaliana by detecting rpoD mRNA using a quantitative reverse transcription-polymerase chain reaction (qRT-PCR). rpoD is a representative housekeeping gene that encodes the principal RNA polymerase sigma factor. Quantification of rpoD mRNA by qRT-PCR allowed easy detection of viability and development of the pathogens in infected plant tissue. Therefore, qRT-PCR technology provides new research opportunities for investigation of host-pathogen interactions.