Priming with Humic Acid to Reverse Ageing Damage in Soybean [Glycine max (L.) Merrill.] Seeds
Priming with Humic Acid to Reverse Ageing Damage in Soybean [Glycine max (L.) Merrill.] Seeds
复制标题
用腐植酸进行底涂可逆转大豆 [Glycine max (L.) Merrill.] 种子的老化损伤
DOI：
10.3390/agriculture11100966
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发表时间：
2021
期刊：
影响因子：
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通讯作者：
M. Ghazali
中科院分区：
文献类型：
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作者：
Indika Weerasekara;U. Sinniah;P. Namasivayam;Muhamad Hazim Nazli;Sharif Azmi Abdurahman;M. Ghazali
大豆种子活力随着贮藏时间的延长而下降，这是由于老化，这可以通过种子引发来缓解。在室内和温室条件下，研究了腐殖酸（HA）引发对玉米种子萌发、活力和幼苗生长的影响。在25 °C下，用0.2g/L HA溶液或蒸馏水（水引发）引发具有60%发芽率的储存12个月的种子1、3、5和7小时。未引发的干种子用作对照，得到9种处理组合。各种发芽性状[平均发芽时间（MGT）、最终发芽率（FG%）、发芽率指数（GRI）、出苗率（SEP）]、平均出苗时间（MET）、幼苗质量性状[幼苗活力指数（SVI）、芽长、根长、根体积]、抗氧化酶活性[过氧化氢酶（CAT）、过氧化物酶（POD）]、测定脂质过氧化[丙二醛（MDA）]和电导率（EC）。室内发芽试验采用单因素（9个引发处理），温室试验采用双因素（2个土壤水分水平（50和80%FC）和9个引发处理）。结果表明，与未引发的种子相比，HA引发5 h后，CAT活性、POD活性分别恢复了25%、50%，EC、MDA含量分别降低了51%、40%，MET降低了25%、50%、40%。在50%FC和80%FC水平下，FG%、GRI和SEP分别从4.3天增加到71%、15.6-21.1和35 - 54%和60 - 72%。MDA含量与GRI呈极显著负相关（r =-0.80 **），CAT和POD活性与GRI呈显著正相关（r= 0.67 ** 和r = 0.56 **）。因此，引发与0.2 g/L HA为5小时，改善了最低限度恶化大豆种子的活力，从而导致更均匀的田间建立增加出苗。
Soybean seed vigour declines with increase in storage duration, due to ageing, which can be alleviated through seed priming. The objective of this study was to investigate the effects of Humic acid (HA) priming on germination, vigour and seedling performance under laboratory and greenhouse conditions with two soil moisture level [50% and 80% field capacity (FC)]. Seeds stored for 12 months having 60% germination were primed either with 0.2 g/L HA solution or distilled water (hydro-primed) at 25 °C for 1, 3, 5 and 7 h. Non-primed dry seeds were used as control, giving nine treatment combinations. Various germination traits [mean germination time (MGT), final germination percentage (FG%), germination rate index (GRI), seedling emergence percentage (SEP)], mean emergence time (MET), seedling quality traits [seedling vigor index (SVI), shoot length, root length, root volume], antioxidant enzyme activities [catalase (CAT), peroxidase (POD)], lipid peroxidation [malondialdehyde (MDA)] and electrical conductivity (EC) were determined. A germination test in the laboratory was conducted as single factor (nine priming treatments), while the greenhouse experiment was conducted as two factors [2 soil moisture level (50 and 80% FC) and 9 priming treatments]. The results indicated that seeds primed with HA for 5 h was able to reinstate the CAT activities (25%), POD activities (50%) and reduced EC (51%) and MDA content (40%) compared with non-primed seeds, reduced the MET (from 4.3 to 3.5 days), increased FG% (from 62 to 71%), GRI (15.6–21.1) and SEP (from 35 to 54%) and (from 60 to 72%) at 50% FC level and 80% FC level, respectively. A strong negative correlation (r = −0.80 **) was found between MDA content and GRI, while CAT and POD activities had positive correlation with GRI r= 0.67 ** and r = 0.56 **, respectively. Thus, priming with 0.2 g/L HA for 5 h improved the vigour of minimally deteriorated soybean seeds resulting in increased emergence with more uniform field establishment.