Germline transcription: a key regulator of accessibility and recombination.
Germline transcription: a key regulator of accessibility and recombination.
复制标题
种系转录：可及性和重组的关键调节因子。
DOI：
10.1007/978-1-4419-0296-2_8
复制
发表时间：
2009
影响因子：
--
通讯作者：
Krangel,MichaelS
中科院分区：
文献类型：
--
作者：
Abarrategui,Iratxe;Krangel,MichaelS
V（D）J重组的发育调控是在重组信号序列（recombination signal sequences，RSSs）与重组酶的染色质可接近性水平上进行的，启动子和增强子等顺式转录调控元件在体内可接近性调控中起着重要作用。然而，这些元素影响可及性的分子机制仍在研究中。虽然V（D）J重组的可及性通常伴随着抗原受体基因座处的种系转录，但这种转录在指导RSS可及性中的功能意义一直是难以捉摸的。在这一章中，我们回顾了过去的研究概述了V（D）J重组和转录之间的复杂关系，以及我们目前的理解，染色质结构是如何在基因表达过程中进行调节。然后，我们总结了最近的工作，直接解决转录的功能作用，在V（D）J重组。
The developmental control of V(D)J recombination is imposed at the level of chromatin accessibility of recombination signal sequences (RSSs) to the recombinase machinery.Cis-acting transcriptional regulatory elements such as promoters and enhancers play a central role in the control of accessibility in vivo. However, the molecular mechanisms by which these elements influence accessibility are still under investigation. Although accessibility for V(D)J recombination is usually accompanied by germline transcription at antigen receptor loci, the functional significance of this transcription in directing RSS accessibility has been elusive. In this chapter, we review past studies outlining the complex relationship between V(D)J recombination and transcription as well as our current understanding on how chromatin structure is regulated during gene expression. We then summarize recent work that directly addresses the functional role of transcription in V(D)J recombination.