Precise annotation of human, chimpanzee, rhesus macaque and mouse mitochondrial genomes leads to insight into mitochondrial transcription in mammals
Precise annotation of human, chimpanzee, rhesus macaque and mouse mitochondrial genomes leads to insight into mitochondrial transcription in mammals
复制标题
人类、黑猩猩、恒河猴和小鼠线粒体基因组的精确注释有助于深入了解哺乳动物的线粒体转录
DOI：
10.1080/15476286.2019.1709746
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发表时间：
2020-01-12
期刊：
影响因子：
4.1
通讯作者：
Gao, Shan
中科院分区：
文献类型：
--
作者：
Jin, Xiufeng;Cheng, Zhi;Gao, Shan
在本研究中，我们使用5‘端和3’端小RNA对人类、黑猩猩、恒河猴和小鼠的线粒体(MT)基因组进行了精确注释。我们的新批注更新了以前的批注。特别是，我们的新注释导致了两个重要的新发现：(1)在控制区识别了5个保守的序列块(CSB1、CSB2、CSB3、LSP和HSP)；(2)对转录起始和新的转录终止位点进行了注释。基于这些注释，我们提出了一个新的mt转录模型，该模型可以解释哺乳动物中mt的转录及其调控。根据我们的模型，转录终止位点作为开关来调节短、长初级转录和不间断转录的产生，而不是简单地终止mt转录。此外，线粒体转录终止因子的表达水平控制着rRNAs、mRNAs和lncRNAs在总mtRNA中的比例。我们的发现指出，哺乳动物中还存在许多其他尚未确定的转录终止位点。
In the present study, we applied our 'precise annotation' to the mitochondrial (mt) genomes of human, chimpanzee, rhesus macaque and mouse using 5 ' and 3 ' end small RNAs. Our new annotations updated previous annotations. In particular, our new annotations led to two important novel findings: (1) the identification of five Conserved Sequence Blocks (CSB1, CSB2, CSB3, LSP and HSP) in the control regions; and (2) the annotation of Transcription Initiation and novel Transcription Termination Sites. Based on these annotations, we proposed a novel model of mt transcription which can account for the mt transcription and its regulation in mammals. According to our model, Transcription Termination Sites function as switches to regulate the production of short, long primary transcripts and uninterrupted transcription, rather than simply terminate the mt transcription. Moreover, the expression levels of mitochondrial transcription termination factors control the proportions of rRNAs, mRNAs and lncRNAs in total mt RNA. Our findings point to the existence of many other, as yet unidentified, Transcription Termination Sites in mammals.