Degradation of arylarsenic compounds by microorganisms
Degradation of arylarsenic compounds by microorganisms
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DOI：
10.1111/j.1574-6968.2007.00835.x
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发表时间：
2007-09-01
影响因子：
2.1
通讯作者：
Morita, Masatoshi
中科院分区：
文献类型：
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作者：
Nakamiya, Kunichika;Nakayama, Takashi;Morita, Masatoshi
当被二苯胂酸 (DPAA) 污染至约 0.5 mM 的土壤用作唯一碳源时，微生物不会直接积累。然而，使用甲苯作为碳源产生了几种分离物，然后将其用于以 DPAA 作为唯一碳源的培养。通过这些方法，分离出可在高达 0.038 mM DPAA 中生长的久坐球菌菌株 NK0508。 DPAA 的毒性阻碍了 K.sentarius 的生长以及环境样品中利用 DPAA 的微生物的直接积累。该菌株可以利用约80%的DPAA和苯胂酸作为唯一碳源3天。 DPAA的降解产物经测定为顺式、顺式、粘康酸和砷酸。当用甲基苯胂酸和二苯甲基胂培养 K.sedentarius 时，观察到两种化合物分别降解约 90% 和 10%。二苯基甲基胂氧化物可能是由 DPAA 甲基化合成的，被检测为转化产物之一。这些结果表明，降解是通过苯基从芳基胂化合物中分裂开始的，随后苯基的羟基化和开环产生顺式、顺式、粘康酸酯。
Microorganisms were not directly accumulated when soil contaminated to about 0.5 mM with diphenylarsinic acid (DPAA) was used as the sole source of carbon. However, using toluene as the carbon source yielded several isolates, which were then used in cultivation with DPAA as the sole source of carbon. By these methods, Kytococcus sedentarius strain NK0508, which can grow in up to 0.038 mM DPAA, was isolated. The toxicity of DPAA retarded the growth of K. sedentarius and the direct accumulation of DPAA-utilizing microorganisms from environmental samples. This strain can utilize about 80% of DPAA and phenylarsonic acid as the sole source of carbon for 3 days. Degradation products of DPAA were determined to be cis, cis, muconate and arsenic acid. When K. sedentarius was cultivated with methylphenylarsinic acid and diphenylmethylarsine, about 90% and 10% degradation of the two compounds, respectively, were observed. Diphenylmethylarsine oxide, possibly synthesized by methylation of DPAA, was detected as one of the transformation products. These results suggest that degradation is initiated by splitting of the phenyl groups from the arylarsenic compounds with subsequent hydroxylation of the phenyl groups and ring opening to yield cis, cis, muconate.