3-DIMENSIONAL ORDERED DISTRIBUTION OF CRYSTALS IN TURKEY TENDON COLLAGEN-FIBERS
3-DIMENSIONAL ORDERED DISTRIBUTION OF CRYSTALS IN TURKEY TENDON COLLAGEN-FIBERS
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DOI：
10.1073/pnas.86.24.9822
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发表时间：
1989-12-01
影响因子：
11.1
通讯作者：
WEINER, S
中科院分区：
文献类型：
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作者：
TRAUB, W;ARAD, T;WEINER, S
用电子显微镜和电子衍射研究了矿化火鸡腱中磷灰石晶体和胶原纤维的组织结构。为了最大限度地减少人为失真的组织进行了检查，第一次，作为孤立的原纤维在玻璃冰的水性环境中，以及在常规制备的部分。电子显微照片显示，板状磷灰石晶体在胶原纤维上平行排列。这为胶原纤维中高度不对称的组装提供了直接证据，事实上，观察到纤维的横截面是细长的而不是圆形的。此外，在相邻的原纤维中，晶体层有明显的一致排列的趋势。这些观察结果对于在分子水平上理解骨的力学行为也可能是重要的，因为这种延伸的、对齐的扁平晶体聚集体可以发展成成熟骨中的自然断裂面。
The organization of apatite crystals and collagen fibrils in mineralized turkey tendon has been studied by electron microscopy and electron diffraction. To minimize artifactual distortions the tissue was examined, for the first time, as isolated fibrils in an aqueous environment of vitreous ice, as well as in conventionally prepared sections. The electron micrographs show that the plate-shaped apatite crystals are arranged in parallel arrays across the collagen fibrils. This provides direct evidence for highly asymmetric assembly in collagen fibrils, and, indeed, the fibrils wer observed to be elongated rather than round in cross-section. There is, furthermore, a pronounced tendency for the layers of crystals to be coherently aligned in adjacent fibrils. These observations may also be important for understanding the mechanical behavior of bone at the molecular level, as such extended, aligned aggregates of flat crystals could develop into natural fracture planes in mature bone.