Promiscuous mitochondria in Cryptococcus gattii
Promiscuous mitochondria in Cryptococcus gattii
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DOI：
10.1111/j.1567-1364.2009.00494.x
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发表时间：
2009-05-01
影响因子：
3.2
通讯作者：
Boekhout, Teun
中科院分区：
文献类型：
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作者：
Bovers, Marjan;Hagen, Ferry;Boekhout, Teun
格特隐球菌是一种主要的致病性担子菌酵母，包括四个基因型组。在这里，我们提出了两个线粒体基因座（MtLrRNA和ATP 6）的数据。两个基因型组，即扩增片段长度多态性（AFLP）5/VGIII和AFLP 6/VGII，形成单系谱系。然而，AFLP 4/VGI基因型组具有五种不同的线粒体基因型，没有形成单系谱系。这些分离株中的大多数含有与AFLP 6/VGII分离株中发现的线粒体基因组部分相同的线粒体基因组，AFLP 6/VGII是导致温哥华岛疫情持续和扩大的原因。4株AFLP 7/VGIV分离株中有2株含有MtLrRNA的AFLP 4/VGI等位基因。这些观察结果最好通过假设线粒体重组过程来解释。如果这是真的，线粒体重组似乎可能属于不同基因型组的细胞之间的C。Gattii，特别是AFLP 6/VGII或AFLP 7/VGIV和AFLP 4/VGI之间。我们还必须假设线粒体很可能是从AFLP 6/VGII细胞转移到AFLP 4/VGI细胞的。由于这种线粒体重组过程仅在细胞-细胞接合后才可能，因此这也可以允许在不同基因型的C.加提。这可能是相关的，因为它可能提供了一种可能的机制，有助于调节分离株的毒力属性，例如在正在进行的温哥华岛C.加提。
Cryptococcus gattii is a primary pathogenic basidiomycetous yeast comprising four genotypic groups. Here we present data on two mitochondrial loci (MtLrRNA and ATP6). Two of the genotypic groups, namely amplified fragment length polymorphism (AFLP)5/VGIII and AFLP6/VGII, formed monophyletic lineages. The AFLP4/VGI genotypic group, however, possessed five different mitochondrial genotypes that did not form a monophyletic lineage. The majority of these isolates contained mitochondrial genomes that are partially identical to those found in isolates belonging to AFLP6/VGII, which is causing the ongoing and expanding Vancouver Island outbreak. Two out of four AFLP7/VGIV isolates contained an AFLP4/VGI allele of MtLrRNA. These observations are best explained by assuming a process of mitochondrial recombination. If this is true, mitochondrial recombination seems possible between cells belonging to different genotypic groups of C. gattii, especially between AFLP6/VGII or AFLP7/VGIV and AFLP4/VGI. We also have to assume that mitochondria, most likely, were transferred from cells belonging to AFLP6/VGII to AFLP4/VGI. As such a process of mitochondrial recombination is only possible after cell-cell conjugation, this may also allow the further exchange of genetic material, for example nuclear or plasmid in nature, between different genotypes of C. gattii. This may be relevant as it may provide a possible mechanism contributing to the modulation of virulence attributes of isolates, such as has been observed in the ongoing Vancouver Island outbreak of C. gattii.