The solubilization and biological activities of Aspergillus β-(1→3)-D-glucan
The solubilization and biological activities of Aspergillus β-(1→3)-D-glucan
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DOI：
10.1016/j.femsim.2004.04.004
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发表时间：
2004-10-01
影响因子：
--
通讯作者：
Ohno, N
中科院分区：
文献类型：
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作者：
Ishibashi, K;Miura, NN;Ohno, N
我们最近已经证明，白念珠菌的细胞壁P-葡聚糖可以溶解次氯酸钠，然后用二甲基亚砜提取（NaClO-DMSO方法）。在这项研究中，将这种方法应用于曲霉属，制备了菌丝体细胞壁β-葡聚糖，并对其物理性质和免疫毒理活性进行了研究。丙酮干燥的曲霉菌丝体。用NaClO-DMSO法氧化。C-13 NMR光谱分析表明，制剂由α-（1 -> 3）和β-（1 -> 3）-D-葡聚糖组成。此外，α-（1 -> 3）和β-（1 -> 3）-D-葡聚糖的比例也不同。以OX-Asp为原料，经尿素自分解制备了可溶性曲霉β-葡聚糖（ASBG）。ASBG显示出与假丝酵母增溶β-葡聚糖（CSBG）相似的鲎活性，并且不同曲霉属之间ASBG的活性差异不大。ASBG影响人外周血单个核细胞（PBMC）产生IL-8。ASBG应有助于分析β-葡聚糖的临床作用。(C)2004年，欧洲微生物学会联合会。Elsevier B. V.出版，保留所有权利。
We have recently demonstrated that the cell wall P-glucan of Candida albicans could be solubilized by sodium hypochlorite, followed by dimethylsulfoxide-extraction (NaClO-DMSO method). In this study, applying this method to Aspergillus spp., we prepared mycelial cell wall P-glucan and examined its physical properties and immuno toxicological activity. The acetone-dried mycelia of Aspergillus spp. were oxidized by the NaClO-DMSO method. An analysis of C-13 NMR spectra revealed the preparations to be composed of alpha-(1 --> 3) and beta-(1 --> 3)-D-glucan. Also, the proportion of alpha-(1 --> 3) and beta-(1 --> 3)-D-glucan varied. Futhermore, a solubilized Aspergillus beta-glucan (ASBG) was prepared from OX-Asp by urea-autocalve treatment. ASBG showed limulus activity similar to Candida solubilized beta-glucan (CSBG), and there was little difference in the activity of ASBG between various Aspergillus spp. ASBG affected the production of IL-8 by human peripheral blood mononuclear cells (PBMC). ASBG should be useful for analyzing the clinical role of beta-glucan. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.