An optical FRET inhibition sensor for serum ferritin based on Mn2+-doped NaYF4:Yb,Tm NIR luminescence up-conversion nanoparticles
An optical FRET inhibition sensor for serum ferritin based on Mn2+-doped NaYF4:Yb,Tm NIR luminescence up-conversion nanoparticles
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基于Mn2+掺杂NaYF4:Yb,Tm近红外发光上转换纳米粒子的血清铁蛋白光学FRET抑制传感器
DOI：
10.1016/j.jlumin.2015.07.048
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发表时间：
2015-12
影响因子：
3.6
通讯作者：
Wang, Lun
中科院分区：
文献类型：
--
作者：
Wang, ShaoZhen;Zhang, Jianguo;Chen, Hongqi;Wang, Lun
A new serum ferritin (SF) biosensor based on fluorescence resonance energy transfer (FRET) from Mn2+-doped NaYF4:Yb,Tm up-converting rare-earth nanophosphors (UCNPs, donor) to gold nanorods (GNRs, acceptor) was developed in the near-infrared (NIR) region. The as-prepared Mn2+-doped NaYF4:Yb,Tm UCNPs exhibited a strong emission at 807 nm in the NIR region and were modified with poly-(acrylic acid) (PAA) before conjugating with anti-SF (Ab1). The negatively charged donor (Ab1-UCNPs) and the positively charged acceptor (GNRs) were mixed in close proximity due to electrostatic interactions, which quenched the fluorescence emission of the Mn2+-doped NaYF4:Yb,Tm UCNPs. However, added SF combines with the anti-SF because the binding affinity between SF and Ab1is stronger than the electrostatic interactions. Thus, the acceptor separated from the donor, and the fluorescence was restored. Under optimal conditions, the restored fluorescence exhibited a linear relationship with SF concentrations ranging from 0.23 to 16.88 ng/mL and a limit of detection as low as 0.12 ng/mL. The proposed method was also used to monitor SF in human serum samples.
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影响因子：
3.9
作者：
Jiang, Shan;Zhang, Yong
通讯作者：
Zhang, Yong
影响因子：
4.2
作者：
N. Milman;L. Pedersen
通讯作者：
N. Milman;L. Pedersen
DOI：
--
发表时间：
--
期刊：
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影响因子：
--
作者：
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通讯作者：
Yan-Mei Wu;Yao Cen;Lijiao Huang;R. Yu;X. Chu
影响因子：
3.7
作者：
Pichaandi, Jothirmayanantham;Boyer, John-Christopher;van Veggel, Frank C. J. M.
通讯作者：
van Veggel, Frank C. J. M.
基于近红外响应 NaYF4:Yb、Er 上转换荧光纳米粒子和金纳米粒子之间的发光共振能量转移的山羊抗人免疫球蛋白 G 抗体的免疫测定。
影响因子：
7.4
作者：
Wang, Meng;Hou, Wei;Xu, Shu-Kun
通讯作者：
Xu, Shu-Kun