Exogenous Neural Stem Cells Transplantation as a Potential Therapy for Photothrombotic Ischemia Stroke in Kunming Mice Model
Exogenous Neural Stem Cells Transplantation as a Potential Therapy for Photothrombotic Ischemia Stroke in Kunming Mice Model
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DOI：
10.1007/s12035-016-9740-6
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发表时间：
2017-03-01
影响因子：
5.1
通讯作者：
Ren, Haijun
中科院分区：
文献类型：
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作者：
Hou, Boru;Ma, Junning;Ren, Haijun
中风被认为是全球第二大死亡原因。中风幸存者会经历不同程度的脑功能损伤，导致衰弱性残疾。目前的中风治疗主要是姑息性的，可能仅对一小部分中风患者有效。在这项研究中，我们探索了外源神经干细胞（NSC）移植作为昆明小鼠模型光血栓缺血性中风的潜在治疗方法。中风后，接受 NSC 移植的小鼠在神经行为测试中表现出更好的脑功能恢复。对 NSC 移植小鼠大脑样本的组织学分析表明，中风引起的脑损伤有所减少。此外，对脑切片中生物标志物的免疫荧光测定证实，移植的 NSC 确实分化为神经元和星形胶质细胞，这与中风后脑功能的改善一致。综上所述，我们的数据表明，外源性 NSC 移植可能是治疗中风的一种有前途的疗法。
Stroke is considered as the second leading cause of death worldwide. The survivors of stroke experience different levels of impairment in brain function resulting in debilitating disabilities. Current therapies for stroke are primarily palliative and may be effective in only a small population of stroke patients. In this study, we explore the transplantation of exogenous neural stem cells (NSCs) as the potential therapy for the photothrombotic ischemia stroke in a Kunming mice model. After stroke, mice receiving NSC transplantation demonstrated a better recovery of brain function during the neurobehavioral tests. Histology analysis of the brain samples from NSC transplanted mice demonstrated a reduction of brain damage caused by stroke. Moreover, immunofluorescence assay for biomarkers in brain sections confirmed that transplanted NSCs indeed differentiated to neurons and astrocytes, consistent with the improved brain function after stroke. Taken together, our data suggested that exogenous NSC transplantation could be a promising therapy for stroke.