Recognizing a single base in an individual DNA strand:: A step toward DNA sequencing in nanopores
Recognizing a single base in an individual DNA strand:: A step toward DNA sequencing in nanopores
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DOI：
10.1002/anie.200462114
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发表时间：
2005-01-01
影响因子：
16.6
通讯作者：
Ghadiri, MR
中科院分区：
文献类型：
--
作者：
Ashkenasy, N;Sánchez-Quesada, J;Ghadiri, MR
单分子水平的功能超分子化学。单链DNA可被α-溶血素跨膜孔蛋白捕获形成单种α-HL·DNA伪轮烷。该过程可用于通过α-HL离子电导的特征性降低来鉴定DNA链上特定位置处的单个腺嘌呤核苷酸。这项研究表明，α-HL介导的单分子DNA测序可能是基本可行的。
Functional supramolecular chemistry at the single-molecule level. Single strands of DNA can be captured inside α-hemolysin transmembrane pore protein to form single-species α-HL·DNA pseudorotaxanes. This process can be used to identify a single adenine nucleotide at a specific location on a strand of DNA by the characteristic reductions in the α-HL ion conductance. This study suggests that α-HL-mediated single-molecule DNA sequencing might be fundamentally feasible.