The protective effect of hypotaurine and cysteine sulphinic acid on peroxynitrite-mediated oxidative reactions
The protective effect of hypotaurine and cysteine sulphinic acid on peroxynitrite-mediated oxidative reactions
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DOI：
10.1080/10715760801999727
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发表时间：
2008-01-01
影响因子：
3.3
通讯作者：
Pecci, Laura
中科院分区：
文献类型：
--
作者：
Fontana, Mario;Giovannitti, Federica;Pecci, Laura
亚牛磺酸（HTAU）和半胱氨酸亚磺酸（CSA）对过氧亚硝基阴离子介导的氧化损伤的保护活性已通过监测不同的靶分子，即酪氨酸、二氢罗丹明-123（DHR）和谷胱甘肽（GSH）来评估。HTAU和CSA在存在和不存在碳酸氢根的情况下对酪氨酸氧化的抑制作用可以归因于它们对羟基（（OH）-O-中心点）和碳酸根（CO 3中心点）自由基的抑制能力。HTAU和CSA也减少酪氨酰基自由基，表明亚磺酸盐的这种修复功能可能作为酪氨酸氧化的额外抑制机制起作用。在过氧亚硝基依赖的氧化反应中，HTAU的抑制作用低于CSA。此外，虽然HTAU和CSA竞争性地抑制GSH的直接氧化过氧亚硝酸盐，HTAU是再次对氧化GSH介导的过氧亚硝酸盐衍生的自由基的效果差。可能参与的次级反应，这可以解释HTAU和CSA的抗氧化活性的差异，进行了讨论。
The protective activity of hypotaurine (HTAU) and cysteine sulphinic acid (CSA) on peroxynitrite-mediated oxidative damage has been assessed by monitoring different target molecules, i.e. tyrosine, dihydrorhodamine-123 (DHR) and glutathione (GSH). The inhibition of tyrosine oxidation exerted by HTAU and CSA both in the presence and the absence of bicarbonate can be ascribed to their ability to scavenge hydroxyl ((OH)-O-center dot) and carbonate (CO3 center dot-) radicals. HTAU and CSA also reduce tyrosyl radicals, suggesting that this repair function of sulphinates might operate as an additional inhibiting mechanism of tyrosine oxidation. In the peroxynitrite-dependent oxidation of DHR, the inhibitory effect of HTAU was lower than that of CSA. Moreover, while HTAU and CSA competitively inhibited the direct oxidation of GSH by peroxynitrite, HTAU was again poorly effective against the oxidation of GSH mediated by peroxynitrite-derived radicals. The possible involvement of secondary reactions, which could explain the difference in antioxidant activity of HTAU and CSA, is discussed.