Lipid domain association of influenza virus proteins detected by dynamic fluorescence microscopy techniques
Lipid domain association of influenza virus proteins detected by dynamic fluorescence microscopy techniques
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DOI：
10.1111/cmi.12045
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发表时间：
2013-02
影响因子：
3.4
通讯作者：
M. Veit;S. Engel;B. Thaa;Silvia Scolari;A. Herrmann
中科院分区：
文献类型：
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作者：
M. Veit;S. Engel;B. Thaa;Silvia Scolari;A. Herrmann
流感病毒被认为是在质膜的筏状结构域中聚集，但许多结论是基于（有争议的）Triton提取实验。在这里，我们回顾了复杂的荧光显微镜方法，如FPALM， FRET和FRAP，有助于我们了解病毒蛋白HA和M2的脂质结构域关联。根据目前的病毒组装和脂质结构域组织模型，总结了这些结果。最后，本文描述了在转染细胞中控制结构域关联的信号如何影响流感病毒的复制。
Influenza virus is thought to assemble in raft domains of the plasma membrane, but many of the conclusions were based on (controversial) Triton extraction experiments. Here we review how sophisticated methods of fluorescence microscopy, such as FPALM, FRET and FRAP, contributed to our understanding of lipid domain association of the viral proteins HA and M2. The results are summarized in light of the current model for virus assembly and lipid domain organization. Finally, it is described how the signals that govern domain association in transfected cells affect replication of influenza virus.