In vitro plant regeneration from decapitated embryonic axes of Clitoria ternatea L.-An important medicinal plant
In vitro plant regeneration from decapitated embryonic axes of Clitoria ternatea L.-An important medicinal plant
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DOI：
10.1016/j.indcrop.2011.07.008
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发表时间：
2012-01-01
影响因子：
5.9
通讯作者：
Tiwari, Kavindra Nath
中科院分区：
文献类型：
--
作者：
Singh, Jayanti;Tiwari, Kavindra Nath
采用去头胚轴（DEAs）为外植体，对球花豆进行了离体克隆繁殖研究。外植体在含细胞分裂素的培养基上诱导出丛生芽。测定了几种细胞分裂素[6-苄氨基嘌呤（BAP）、6-糠基氨基嘌呤（KIN）和噻苯隆（TDZ）]。最好的反应是达到2毫克升（-1）BAP，其中100%的文化产生6.0 +/- 0.14芽每个外植体。MS + 1 mg l（-1）赤霉素（GA（3））最适合芽的伸长。再生的芽生根在半强度Murashige和Skoog（MS）培养基与0.2毫克l（-1）吲哚-3-丁酸（IBA）。小植株在土壤中成功地驯化和建立，并且它们在形态上与源植物难以区分。试管苗达到成熟，正常开花。本文报道的高效再生方案为该植物的微繁殖提供了一种重要的方法。此外，该方案可用于遗传转化这种宝贵的药用植物，其进一步改善。(C)2011 Elsevier B. V.保留所有权利。
In vitro clonal propagation of Clitoria ternatea has been achieved by employing decapitated embryonic axes (DEAs) explants. The explants induced multiple shoots on cytokinin-containing medium. Several cytokinins [6-benzylaminopurine (BAP), 6-furfuryl aminopurine (KIN) and thidiazuron (TDZ)] were assayed. The best response was achieved with 2 mg l(-1) BAP in which 100% of cultures produced 6.0 +/- 0.14 shoots per explant. MS + 1 mg l(-1) gibberellic acid (GA(3)) was the most suitable for shoot elongation. Regenerated shoots were rooted in half-strength Murashige and Skoog (MS) medium with 0.2 mg l(-1) indole-3-butyric acid (IBA). Plantlets were successfully acclimatized and established in soil, and they were morphologically indistinguishable from the source plant. The plantlets attained maturity and flowered normally. The efficient regeneration protocol reported here provides an important method of micropropagation of this plant. Furthermore, this protocol may be used for genetic transformation of this valuable medicinal plant for its further improvement. (C) 2011 Elsevier B.V. All rights reserved.