Effect of Gypenosides on the composition of gut microbiota and metabolic activity in the treatment of CCl4-induced liver injury in rats
Effect of Gypenosides on the composition of gut microbiota and metabolic activity in the treatment of CCl4-induced liver injury in rats
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DOI：
10.1016/j.jff.2022.105233
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发表时间：
2022-10
影响因子：
5.6
通讯作者：
Pengyao Tian;Yu Chen;Jiarong Hang;Ruonan Yu;Chunjie Zhao;Min Zhao;Miao Wang
中科院分区：
文献类型：
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作者：
Pengyao Tian;Yu Chen;Jiarong Hang;Ruonan Yu;Chunjie Zhao;Min Zhao;Miao Wang
长期慢性肝损伤可导致肝硬化或肝功能衰竭的严重后果，因此寻找有效的治疗药物至关重要。绞股蓝总皂苷（Gypenosides，GPs）已被证明对多种肝脏疾病具有治疗作用。为了提供用于治疗肝损伤的GP的总体描述，我们基于代谢组学分析和16 S rRNA高通量测序对肠道微生物群和宿主代谢进行了联合研究。结果表明，GPs治疗可显著改善肝损伤引起的肝功能指标、炎症指标、肠屏障指标和氧化损伤指标的紊乱。H&E染色结果显示，GPs治疗能明显减轻大鼠肝损伤。代谢组学分析在粪便、血浆和尿液中分别鉴定出25、27和31种内源性代谢物。肠道微生物群分析显示，模型组的多样性增加，组间差异显著。Metastats分析确定了13个差异属。斯皮尔曼相关分析发现，肝损伤相关的病理指标或代谢产物的增加与相对丰度增加的属呈正相关，而相对丰度降低的属与它们呈负相关。上述结果表明，GP调节肠道微生物群生态失调-代谢活性紊乱以实现肝损伤的治疗。
Long-term chronic liver damage can lead to serious consequences of cirrhosis or liver failure, so it is crucial to find effective therapeutic drugs. Gypenosides (GPs) has been proved to have therapeutic effects on various liver diseases. To provide an overall description of GPs for the treatment of liver injury, we conducted a combined study of gut microbiota and host metabolism based on metabolomic analysis and 16S rRNA high-throughput sequencing. The results showed that GPs treatment significantly improved the disorder of liver function index, inflammatory index, intestinal barrier index and oxidative damage index induced by liver injury. H&E staining showed that GPs treatment could significantly alleviate liver injury in rats. Metabolomics analysis identified 25, 27 and 31 endogenous metabolites in feces, plasma and urine, respectively. Gut microbiota analysis revealed increased diversity in the model group and significant differences between groups. Metastats analysis identified 13 differential genera. Spearman’s correlation analysis was found that the increase of liver injury-related pathological indices or metabolite showed positive correlation with the genera with increased relative abundance, while the genera with decreased relative abundance showed negative correlation with them. The above results suggest that GPs modulates gut microbiota dysbiosis-metabolic activity disorder to achieve the treatment of liver injury.