STUDIES ON GENETIC CONTROL OF TRYPTOPHAN PYRROLASE IN DROSOPHILA-MELANOGASTER
STUDIES ON GENETIC CONTROL OF TRYPTOPHAN PYRROLASE IN DROSOPHILA-MELANOGASTER
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DOI：
10.1007/bf00334435
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发表时间：
1971-01-01
期刊：
影响因子：
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通讯作者：
CHOVNICK, A
中科院分区：
文献类型：
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作者：
BAILLIE, DL;CHOVNICK, A
A reexamination of the role of the vermilion cistron (v: 1–33.0) in the control of tryptophan pyrrolase inDrosophila melanogasteruncovered several, previously unknown features about this enzyme: 1. Crude extracts of Drosophila possess one or more inhibitors which may be removed by Norit treatment during homogenization. 2. Contrary to previous assertion, crude wild type extracts are stimulated by an exogenous source of hematin, and upon purification, become heavily dependent upon added methemoglobin. 3. The wild type enzyme exhibits a lag period in time course of product accumulation which is eliminated by preincubation with methemoglobin and α-methyltryptophan. 4. The molecular weight of the wild type enzyme is estimated at approximately 150000 Daltons. 5. A linear increase of tryptophan pyrrolase activity as a function of dosage ofv+alleles supports the contention that the vermilion cistron is the structural gene for this enzyme. 6. A sex difference in response to gene dosage (dosage compensation) is seen.A suppressor locus (su(s): 1-0.0) is known, whose mutants operate as recessive suppressors of certain vermilion alleles and not others. Experiments are described which bear upon the mode of suppressor action, and a model is offered which is based upon the present data as well as other work reported recently.
