Demonstration of a saturable binding site for thyrotropin in Yersinia enterocolitica.
Demonstration of a saturable binding site for thyrotropin in Yersinia enterocolitica.
复制标题
DOI：
10.1126/science.6298936
复制
发表时间：
1983-03
期刊：
影响因子：
56.9
通讯作者：
M. Weiss;S. Ingbar;S. Winblad;D. Kasper
中科院分区：
文献类型：
--
作者：
M. Weiss;S. Ingbar;S. Winblad;D. Kasper
多项证据表明，人类甲状腺质膜与肠道病原体小肠结肠炎耶尔森氏菌的抗原决定簇之间可能存在免疫交叉反应。因此进行研究以确定小肠结肠炎耶尔森氏菌是否像甲状腺膜一样含有促甲状腺素结合位点。确实证明了牛促甲状腺素的可饱和结合位点，特别是在用乙二胺四乙酸盐和溶菌酶处理过的生物体制剂中。从 125I 标记的牛促甲状腺素的结合抑制来看，结合位点的激素特异性与人甲状腺组织中促甲状腺素受体的相似。
Several lines of evidence suggest that there might be immunologic cross-reactivity between the thyroid plasma membrane in humans and antigenic determinants in the enteric pathogen Yersinia enterocolitica. Studies were therefore performed to determine whether Y. enterocolitica, like the thyroid membrane, contains a thyrotropin binding site. A saturable binding site for bovine thyrotropin was indeed demonstrable, particularly in preparations of the organism that have been treated with ethylenediaminetetraacetate and lysozyme. Hormonal specificity of the binding site, as judged from the inhibition of binding of 125I-labeled bovine thyrotropin, was similar to that of the thyrotropin receptor in human thyroid tissue.