Three-dimensional ultrastructure and histomorphology of mouse circumvallate papillary taste buds before and after birth using focused ion beam-scanning electron microscope tomography
Three-dimensional ultrastructure and histomorphology of mouse circumvallate papillary taste buds before and after birth using focused ion beam-scanning electron microscope tomography
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聚焦离子束扫描电子显微镜断层扫描小鼠出生前后乳头状味蕾的三维超微结构和组织形态学
DOI：
10.1016/j.tice.2021.101714
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发表时间：
2022
期刊：
影响因子：
2.6
通讯作者：
Ohta Keisuke
中科院分区：
文献类型：
--
作者：
Hino Kiyosato;Hirashima Shingo;Tsuneyoshi Risa;Togo Akinobu;Hiroshige Tasuku;Kusukawa Jingo;Nakamura Kei-Ichiro;Ohta Keisuke
早期的味蕾由基板细胞形成。胎盘细胞在出生时分化为I-III型细胞;然而，这些第一味细胞的超微结构仍然难以捉摸。在这里，我们使用聚焦离子束扫描电子显微镜（FIB-SEM）分析味蕾的背表面的轮廓乳头在胚胎天（E）18.5和出生后的天（P）1.5。E18上的味蕾5号以未成熟细胞团存在。其中一个未成熟的细胞从味蕾块延伸到上皮表面的细胞突起。该细胞的细胞质中含有许多线粒体和囊泡的顶端区域。P1的味蕾。5具有小的味孔并且具有洋葱形结构。味蕾中的大多数细胞向味孔延伸。味蕾中的一些细胞是II型样细胞，细胞质中含有糖原。在这项研究中，它是在三维显示，未成熟的细胞延伸到上皮细胞的表面之前形成的味孔。随后，味孔的形成和味蕾的成熟同时进行。
Early taste buds are formed from placode cells. Placode cells differentiate into Type I–III cells at birth; however, the ultrastructure of these first taste cells remain elusive. Here, we used focused ion beam-scanning electron microscopy (FIB-SEM) to analyze taste buds on the dorsal surface of the circumvallate papilla on embryonic day (E) 18.5 and postnatal day (P) 1.5. The taste buds on E18. 5 existed as a mass of immature cells. One of the immature cells extended the cell process to the surface of the epithelium from the taste bud mass. Cytoplasm of this cell contained many mitochondria and vesicles in the apical region. The taste buds at P1. 5 had small taste pores and had an onion-shaped structure. Most of the cells in the taste buds extended toward the taste pores. Some of the cells in the taste buds were Type II-like cells with glycogen in their cytoplasm. In this study, it was shown in three dimensions that immature cells extend to the surface of epithelium before the formation of the taste pore. Subsequently, the formation of taste pores and maturation of taste buds progress simultaneously.