Pore formation of phospholipid membranes by the action of two hemolytic arachnid peptides of different size
Pore formation of phospholipid membranes by the action of two hemolytic arachnid peptides of different size
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DOI：
10.1016/j.bbamem.2004.05.007
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发表时间：
2004-08-30
影响因子：
3.4
通讯作者：
Corzo, G
中科院分区：
文献类型：
--
作者：
Belokoneva, OS;Satake, H;Corzo, G
Pin2和Oxki 1是阳离子两亲性肽，通过形成孔渗透脂质膜。它们与磷酸胆碱（PC）膜结合的机制不同。自旋探针实验表明，Pin2和Oxki1都能穿透小单层囊泡（SUV）的脂质膜。此外，钙黄绿素和葡聚糖从PC囊泡的泄漏表明，Pin2同意肽在脂膜上的积累，并形成不同大小的孔。另一方面，Oxki1没有严格的合作行为，并形成有限大小的孔。(C)2004 Elsevier B.V.保留所有权利。
Pin2 and Oxki1 are cationic amphipathic peptides that permeate lipid membranes through formation of pores. Their mechanism of binding to phosphocholine (PC) membranes differs. Spin-probe experiments showed that both Pin2 and Oxki1 penetrate the lipid membrane of small unilamellar vesicles (SUVs). Moreover, the leakage of calcein and dextrans from PC vesicles showed that Pin2 agrees with the accumulation of peptides on lipid membranes and form pores of different size. On the other hand, Oxki1 did not act strictly cooperatively and form pores of limited size. (C) 2004 Elsevier B.V. All rights reserved.