Comparative and Transcriptome Analyses Uncover Key Aspects of Coding- and Long Noncoding RNAs in Flatworm Mitochondrial Genomes.
Comparative and Transcriptome Analyses Uncover Key Aspects of Coding- and Long Noncoding RNAs in Flatworm Mitochondrial Genomes.
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DOI：
10.1534/g3.116.028175
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发表时间：
2016-05-03
期刊：
影响因子：
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通讯作者：
Sánchez Alvarado A
中科院分区：
文献类型：
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作者：
Ross E;Blair D;Guerrero-Hernández C;Sánchez Alvarado A
利用线粒体基因组的各种特征的保守性有助于解决系统发育关系。尽管有大量的序列证据，但以前还不可能最终解决扁形虫线粒体基因组的一些关键方面，包括通常保守的性状，如起始密码子，非编码区，tRNA的完整互补序列，以及ATP 8是否由该细胞核基因组编码。在努力解决这些困难，我们试图确定线粒体转录组和基因组的有性和无性类群的淡水三叶虫，一组以前在扁形虫有丝分裂基因组学研究代表性差。我们已经发现了一个替代的起始密码子，一个扩展的cox 1基因，一个以前未描述的保守的开放阅读框架，长的非编码RNA，和一个高度保守的基因顺序跨越大的进化距离内的triclads表示的证据。我们的研究结果有助于扩展和完善有丝分裂基因组学，以解决这一多样化动物群体的进化问题。
Exploiting the conservation of various features of mitochondrial genomes has been instrumental in resolving phylogenetic relationships. Despite extensive sequence evidence, it has not previously been possible to conclusively resolve some key aspects of flatworm mitochondrial genomes, including generally conserved traits, such as start codons, noncoding regions, the full complement of tRNAs, and whether ATP8 is, or is not, encoded by this extranuclear genome. In an effort to address these difficulties, we sought to determine the mitochondrial transcriptomes and genomes of sexual and asexual taxa of freshwater triclads, a group previously poorly represented in flatworm mitogenomic studies. We have discovered evidence for an alternative start codon, an extended cox1 gene, a previously undescribed conserved open reading frame, long noncoding RNAs, and a highly conserved gene order across the large evolutionary distances represented within the triclads. Our findings contribute to the expansion and refinement of mitogenomics to address evolutionary issues in this diverse group of animals.