CD18 (beta 2 leukocyte integrin) promoter requires PU.1 transcription factor for myeloid activity.
CD18 (beta 2 leukocyte integrin) promoter requires PU.1 transcription factor for myeloid activity.
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DOI：
10.1073/pnas.92.3.801
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发表时间：
1995-01
影响因子：
11.1
通讯作者：
A. Rosmarin;D. Caprio;R. Levy;C. Simkevich
中科院分区：
文献类型：
--
作者：
A. Rosmarin;D. Caprio;R. Levy;C. Simkevich
正常的细胞分化与严格调控的基因转录有关。然而，调控髓系细胞转录的DNA元件和反式作用因子尚不清楚。CD18是白细胞整合素的β链，在髓系分化过程中受转录调控。CD18启动子在转染髓系细胞后具有活性。我们证明，CD18启动子的一个区域包含PU.1转录因子的两个结合位点，是髓系细胞活性所必需的。这些位点被体外翻译的PU.1和髓核提取物中的PU.1结合。这些位点的突变取消了PU.1的结合，并显著降低了髓系细胞中的启动子活性。因此，CD18的髓系活性需要白细胞特异性转录因子PU.1。
Normal cellular differentiation is linked to tightly regulated gene transcription. However, the DNA elements and trans-acting factors that regulate transcription in myeloid cells are poorly defined. CD18, the beta chain of the leukocyte integrins, is transcriptionally regulated during myeloid differentiation. The CD18 promoter is active after transfection into myeloid cells. We demonstrate that a region of the CD18 promoter that contains two binding sites for the PU.1 transcription factor is required for activity in myeloid cells. These sites are bound by in vitro translated PU.1 and by PU.1 from myeloid nuclear extracts. Mutagenesis of these sites abrogates binding by PU.1 and substantially decreases promoter activity in myeloid cells. Thus, the leukocyte-specific transcription factor PU.1 is required for myeloid activity of CD18.