Inhibition mechanism of lanthanum ion on the activity of horseradish peroxidase in vitro
Inhibition mechanism of lanthanum ion on the activity of horseradish peroxidase in vitro
复制标题
镧离子体外抑制辣根过氧化物酶活性的机制
DOI：
10.1016/j.saa.2009.11.033
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发表时间：
2010-02-01
影响因子：
4.4
通讯作者：
Huang, Xiaohua
中科院分区：
文献类型：
--
作者：
Guo, Shaofen;Wang, Lihong;Huang, Xiaohua
为了了解镧离子（La3+）对辣根过氧化物酶（HRP）活性的抑制机制，采用循环伏安法（CV）研究了La3+对辣根过氧化物酶（HRP）活性、电子转移和体外构象的影响。原子力显微镜（AFM）、圆二色法（CID）、高效液相色谱法（HPLC）、基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF/MS）和电感耦合等离子体质谱法（ICP-MS）。发现La3+可以与HRP分子中多肽链的酰胺基团结合，形成La3+与HRP的复合物（La-HRP）。La-HRP复合物的形成导致HRP分子固有结构的破坏，导致HRP分子血红素基卟啉环的非平面性降低，进而导致HRP分子卟啉环活性中心Fe（III）的暴露程度降低。因此，HRP的直接电化学和催化活性降低。这可能是La3+对过氧化物酶活性的抑制机制。(C) 2009 Elsevier B.V.版权所有
In order to understand the inhibition mechanism of lanthanum ion (La3+) on the activity of horseradish peroxidase (HRP), the effects of La3+ on the activity, electron transfer and conformation of HRP in vitro were investigated by using cyclic voltammetry (CV). atomic force microscopy (AFM), circular dichroism (CID), high performance liquid chromatography (HPLC), matrix-assisted laser desorption/ionization time-of-flight mass spectroscopy (MALDI-TOF/MS) and inductively coupled plasma mass spectrometry (ICP-MS). It was found that La3+ can combine with the amide groups of the polypeptide chain in HRP molecule, forming the complex of La3+ and HRP (La-HRP). The formation of the La-HRP complex causes the destruction of the native structure of HRP molecule, leading to the decrease in the non-planarity of the porphyrin ring in the heme group of HRP molecule, and then in the exposure extent of active center, Fe(III) of the porphyrin ring of HRP molecule. Thus, the direct electrochemical and catalytic activities of HRP are decreased. it is a possible inhibition mechanism of La3+ on the activity of peroxidase. (C) 2009 Elsevier B.V. All rights reserved.