Differential Expression of Complement Proteins in Cerebrospinal Fluid From Active Multiple Sclerosis Patients
Differential Expression of Complement Proteins in Cerebrospinal Fluid From Active Multiple Sclerosis Patients
复制标题
活动性多发性硬化症患者脑脊液中补体蛋白的差异表达
DOI：
10.1002/jcb.23113
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发表时间：
2011-07-01
影响因子：
4
通讯作者：
Liu, Shilian
中科院分区：
文献类型：
--
作者：
Li, Yun;Qin, Zhaoyu;Liu, Shilian
多发性硬化症(MS)是一种具有复杂免疫发病机制的中枢神经系统脱髓鞘疾病。利用二维DGE技术，我们分离了活动期MS患者和对照组的脑脊液蛋白。所鉴定的7个差异蛋白中有3个与补体系统相关，差异蛋白的网络分析显示补体激活参与了活动性MS，经Western blotting分析证实补体C4b(伽马链)升高(P<；0.01)。本研究结果是活动期MS患者脑脊液蛋白质组学的独立定量指标。补体C4b及其相关蛋白在脑脊液中的差异表达为补体激活在MS疾病发病机制中的作用提供了潜在的生物标志物和证据。J.细胞。生物化学。112：1930-1937,2011。(C)2011年Wiley-Liss，Inc.
Multiple sclerosis (MS) is a demyelinating disease of the central nervous system with complex immunopathogenesis. Using the 2-D DIGE technology, we separate CSF proteins from patients with active MS and control subjects. Three of the seven differential proteins identified were related with complement system, and the network analysis of the differential proteins revealed complement activation involvement in active MS. Complement C4b (gamma chain) was confirmed elevated by performing western blotting analysis (P < 0.01). The present results are an independent quantitative proteomic measure in CSF from active MS patients. The differential expression of the complement C4b and related proteins in CSF provides potential biomarkers as well as evidence for the involvement of complement activation in the pathogenesis of MS disease. J. Cell. Biochem. 112: 1930-1937, 2011. (C) 2011 Wiley-Liss, Inc.