Antinociceptive effect of different types of calcium channel inhibitors and the distribution of various calcium channel α1 subunits in the dorsal horn of spinal cord in mice
Antinociceptive effect of different types of calcium channel inhibitors and the distribution of various calcium channel α1 subunits in the dorsal horn of spinal cord in mice
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DOI：
10.1016/j.brainres.2004.07.066
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发表时间：
2004-10-22
期刊：
影响因子：
2.9
通讯作者：
Iijima, T
中科院分区：
文献类型：
--
作者：
Murakami, M;Nakagawasai, O;Iijima, T
为了更好地了解哪些电压依赖性钙通道（VGCC）参与伤害性神经传递，我们研究了 VGCC 在小鼠脊髓中的药理学特性和分布。行为分析显示，鞘内注射分别阻断 P/Q-、P/Q-、N-和 R-型钙通道的 omega-agatoxin TK、omega-agatoxin IVA、omega-conotoxin GVIA 和 SNX-482 产生镇痛作用，而 L-type 通道阻滞剂则没有这种作用。一项电生理学研究表明，背根神经节 (DRG) 神经元内存在各种类型的 VGCC。免疫组织化学揭示了 P/Q、N、L 和 R 型钙通道亚基在脊髓背角的明显定位。这项研究的结果揭示了小鼠脊髓背角内参与伤害性神经传递的几种钙通道的定位和功能。 (C) 2004 Elsevier B.V. 保留所有权利。
To understand better which voltage-dependent calcium channels (VGCCs) are involved in nociceptive neurotransmission, we investigated the pharmacological properties and distribution of VGCCs in the mouse spinal cord. A behavioral assay revealed that intrathecal injections of omega-agatoxin TK, omega-agatoxin IVA, omega-conotoxin GVIA, and SNX-482, which block P/Q-, P/Q-, N-, and R-type calcium channels, respectively, produced analgesic effects, while an L-type channel blocker had no such effect. An electrophysiological study demonstrated the presence of various types of VGCCs within dorsal root ganglion (DRG) neurons. Immunohistochemistry revealed distinct localization of P/Q-, N-, L-, and R-type calcium channel subunits to the dorsal horn of the spinal cord. The results of this study revealed the localization and functions of several calcium channels that are involved in nociceptive neurotransmission within the dorsal horn of the mouse spinal cord. (C) 2004 Elsevier B.V. All rights reserved.