Functional recovery following traumatic spinal cord injury mediated by a unique polymer scaffold seeded with neural stem cells
Functional recovery following traumatic spinal cord injury mediated by a unique polymer scaffold seeded with neural stem cells
复制标题
DOI：
10.1073/pnas.052678899
复制
发表时间：
2002-03-05
影响因子：
11.1
通讯作者：
Snyder, EY
中科院分区：
文献类型：
--
作者：
Teng, YD;Lavik, EB;Snyder, EY
为了更好地直接修复脊髓损伤（SCI）后，我们设计了一种植入物建模后，完整的脊髓组成的多组分聚合物支架与神经干细胞接种。相对于损伤对照组，将支架-神经干细胞单元植入成年大鼠SCI半切模型促进了功能的长期改善（在某些动物中持续1年）。在损伤后70天，植入支架加细胞的动物表现出协调的、负重的后肢行走。组织学和免疫细胞化学分析表明，这种恢复可能部分归因于减少继发性损伤过程中的组织损失以及减少胶质瘢痕形成。束示踪显示皮质脊髓束纤维穿过损伤中心到达尾脊髓，这一现象在未治疗组中不存在。与对照组中未见的增强的局部GAP-43表达的证据一起，这些发现表明可能的再生组分。这些结果可能为SCI提供了一种新的治疗方法，更广泛地说，可以作为针对复杂神经问题的多学科策略的原型。
To better direct repair following spinal cord injury (SCI), we designed an implant modeled after the intact spinal cord consisting of a multicomponent polymer scaffold seeded with neural stem cells. Implantation of the scaffold-neural stem cells unit into an adult rat hemisection model of SCI promoted long-term improvement in function (persistent for 1 year in some animals) relative to a lesion-control group. At 70 days postinjury, animals implanted with scaffold-plus-cells exhibited coordinated, weight-bearing hindlimb stepping. Histology and immunocytochemical analysis suggested that this recovery might be attributable partly to a reduction in tissue loss from secondary injury processes as well as in diminished glial scarring. Tract tracing demonstrated corticospinal tract fibers passing through the injury epicenter to the caudal cord, a phenomenon not present in untreated groups. Together with evidence of enhanced local GAP-43 expression not seen in controls, these findings suggest a possible regeneration component. These results may suggest a new approach to SCI and, more broadly, may serve as a prototype for multidisciplinary strategies against complex neurological problems.