The differential role of human macrophage in triggering secondary bystander effects after either gamma-ray or carbon beam irradiation.
The differential role of human macrophage in triggering secondary bystander effects after either gamma-ray or carbon beam irradiation.
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人巨噬细胞在伽马射线或碳梁照射后触发继发旁观者效应中的差异作用。
DOI：
10.1016/j.canlet.2015.04.013
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发表时间：
2015-07-10
期刊：
影响因子：
9.7
通讯作者：
Shao, Chunlin
中科院分区：
文献类型：
--
作者：
Dong, Chen;He, Mingyuan;Tu, Wenzhi;Konishi, Teruaki;Liu, Weili;Xie, Yuexia;Dang, Bingrong;Li, Wenjian;Uchihori, Yukio;Hei, Tom K.;Shao, Chunlin
远隔效应可能是评估放疗预后的潜在因素。本研究建立了体外系统，检测肿瘤产生的旁观者信号是否可以通过巨噬细胞传递，以进一步触发不同照射后的二次细胞反应，其中人肺癌NCI-H446细胞用γ射线或碳离子照射，并与人巨噬细胞U937细胞共培养，然后将这些U937细胞作为旁观者信号传递者与人支气管上皮细胞BEAS-2B共培养。结果表明，U937细胞仅被γ射线照射的NCI-H446细胞激活，因此碳离子照射下BEAS-2B细胞的继发损伤较γ射线弱。 TNF-α 和 IL-1α 均参与 γ 辐射诱导的次级旁观者效应，但只有 TNF-α 有助于碳离子诱导的反应。进一步的分析表明，IL-1α而不是TNF-α在很大程度上负责BEAS-2B细胞中巨噬细胞的激活和微核的形成。这些数据表明，巨噬细胞可以传递次级旁观者信号，并在光子辐照的次级旁观者效应中发挥关键作用，而碳离子辐照由于减少了次级损伤而具有明显的优势。
The abscopal effect could be an underlying factor in evaluating prognosis of radiotherapy. This study established an in vitro system to examine whether tumor-generated bystander signals could be transmitted by macrophages to further trigger secondary cellular responses after different irradiations, where human lung cancer NCI-H446 cells were irradiated with either γ-rays or carbon ions and co-cultured with human macrophage U937 cells, then these U937 cells were used as a bystander signal transmitter and co-cultured with human bronchial epithelial cells BEAS-2B. Results showed that U937 cells were only activated by γ-irradiated NCI-H446 cells so that the secondary injuries in BEAS-2B cells under carbon ion irradiation were weaker than γ-rays. Both TNF-α and IL-1α were involved in γ-irradiation induced secondary bystander effect but only TNF-α contributed to the carbon ion induced response. Further assay disclosed that IL-1α but not TNF-α was largely responsible for the activation of macrophages and the formation of micronucleus in BEAS-2B cells. These data suggest that macrophages could transfer secondary bystander signals and play a key role in the secondary bystander effect of photon irradiation while carbon ion irradiation has conspicuous advantage due to its reduced secondary injury.
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影响因子：
3.2
作者：
Casey, Dana L.;Friedman, Danielle N.;Wolden, Suzanne L.
通讯作者：
Wolden, Suzanne L.
影响因子：
2.6
作者：
Li, Jitao;He, Mingyuan;Shao, Chunlin
通讯作者：
Shao, Chunlin
影响因子：
11.2
作者：
Lorimore, Sally A.;Chrystal, Jennifer A.;Wright, Eric G.
通讯作者：
Wright, Eric G.
DOI：
10.1016/j.mrfmmm.2009.12.007
发表时间：
2010-04-01
影响因子：
2.3
作者：
Asur, Rajalakshmi;Balasubramaniam, Mamtha;Tucker, James D.
通讯作者：
Tucker, James D.
影响因子：
2
作者：
Autsavapromporn N;De Toledo SM;Buonanno M;Jay-Gerin JP;Harris AL;Azzam EI
通讯作者：
Azzam EI