ADIPOGENIC ACTIVITIES IN COMMERCIAL PREPARATIONS OF FETUIN
ADIPOGENIC ACTIVITIES IN COMMERCIAL PREPARATIONS OF FETUIN
复制标题
DOI：
10.1055/s-2007-1000780
复制
发表时间：
1994-02-01
影响因子：
2.2
通讯作者：
LOFFLER, G
中科院分区：
文献类型：
--
作者：
BACHMEIER, M;LOFFLER, G
胎球蛋白经常被用作血清替代品，特别是在分化前脂肪细胞系的培养过程中。我们在这里表明，市售的粗Pedersen胎球蛋白含有允许脂肪转化的因子。当3 T3-L1细胞在纯化的Pedersen胎球蛋白存在下培养时，它们不能进行脂肪转化，除非将这种允许的脂肪形成因子加入培养基中。胰岛素样生长因子I（IGF-I）和生长激素（GH）不能替代该因子。
Fetuin is frequently in use as a serum substitute especially during the culture of differentiating preadipocyte cell lines. We show here, that the commercially available crude Pedersen fetuin contains a factor permissive for adipose conversion. It can be separated from fetuin by dialysis at acid pH. When 3T3-L1 cells are cultured in the presence of purified Pedersen fetuin, they fail to undergo adipose conversion unless this permissive adipogenic factor is added to the culture medium. Insulin-like growth factor I (IGF-I) and growth hormone (GH) are not able to replace the factor.