A MUTATION IN THE RCC1-RELATED PROTEIN PIM1 RESULTS IN NUCLEAR-ENVELOPE FRAGMENTATION IN FISSION YEAST
A MUTATION IN THE RCC1-RELATED PROTEIN PIM1 RESULTS IN NUCLEAR-ENVELOPE FRAGMENTATION IN FISSION YEAST
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DOI：
10.1073/pnas.92.5.1436
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发表时间：
1995-02-28
影响因子：
11.1
通讯作者：
SAZER, S
中科院分区：
文献类型：
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作者：
DEMETER, J;MORPHEW, M;SAZER, S
RCC 1蛋白家族的成员是染色质相关的鸟嘌呤核苷酸交换因子，其在各种生物体中的不同细胞过程中有牵连，但尚未就其主要生物学作用达成共识。裂殖酵母粟酒裂殖酵母（Schizosophyomycespombe），一种单细胞真核生物，提供了一种体内系统，在该系统中通过使用RCC 1相关蛋白pim 1中的温度敏感突变体来研究RCC 1/Ran开关。pim 1-d1（ts）突变体中的有丝分裂进入是正常的，但是有丝分裂退出导致被中间隔膜和浓缩的染色体阻滞的细胞的积累，虽然酵母核膜在整个细胞周期中通常保持完整，但我们发现在有丝分裂后pim 1-d1（ts）突变体中核膜的显著片段化，这导致染色质不再被区室化，并且在细胞质中积累了含孔膜，这种终末表型的发展依赖于细胞通过有丝分裂的传代，并且与活力的丧失一致。我们建议，pim 1是需要重建核结构有丝分裂酵母。
Members of the RCC1 protein family are chromatin-associated guanine nucleotide exchange factors that have been implicated in diverse cellular processes in various organisms, yet no consensus has been reached as to their primary biological role. The fission yeast Schizosaccharomyces pombe, a single-celled eukaryote, provides an in vivo system in which to study the RCC1/Ran switch by using a temperature-sensitive mutant in the RCC1-related protein pim1, Mitotic entry in the pim1-d1(ts) mutant is normal, but mitotic exit leads to the accumulation of cells arrested with a medial septum and condensed chromosomes, Although the yeast nuclear envelope normally remains intact throughout the cell cycle, we found a striking fragmentation of the nuclear envelope in the pim1-d1(ts) mutant following mitosis, This resulted in chromatin that was no longer compartmentalized and an accumulation of pore-containing membranes in the cytoplasm, The development of this terminal phenotype was dependent on the passage of cells through mitosis and was coincident with the loss of viability. We propose that pim1 is required for the reestablishment of nuclear structure following mitosis in fission yeast.