Pivotal role of capsaicin-sensitive primary afferents in development of both heat and mechanical hyperalgesia induced by intraplantar bee venom injection
Pivotal role of capsaicin-sensitive primary afferents in development of both heat and mechanical hyperalgesia induced by intraplantar bee venom injection
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DOI：
10.1016/s0304-3959(00)00458-9
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发表时间：
2001-04-01
期刊：
影响因子：
7.4
通讯作者：
Chen, HS
中科院分区：
文献类型：
--
作者：
Chen, J;Chen, HS
为探讨初级传入纤维在蜂毒(BV)诱导的持续性自发伤害性感觉(PSN)和痛觉过敏(HA)形成中的作用，在戊巴比妥钠麻醉下分别用辣椒素局部处理大鼠坐骨神经或坐骨神经和隐神经，破坏辣椒素敏感的初级传入纤维(CSPA)。此外，还观察了坐骨神经辣椒素对福尔马林诱导的多发性硬化的影响。损毁坐骨神经或坐骨神经和隐神经的CSPA纤维仅对BV诱导的1h爪缩的平均总数产生34%或69%的抑制。然而，福尔马林诱导的1h足爪皱缩总数被抑制了90%(相1和相2分别为85%和91%)。在幼鼠中，破坏坐骨神经的CSPA纤维导致注射部位(爪垫)和后爪后跟的辐射热反应潜伏期(PWTL)分别比基线值增加237和60%。但对非治疗侧PWTL和双后爪对von Frey细丝刺激的缩爪机械阈值(PWMT)无明显影响。在BV治疗的大鼠中。CSPA纤维对坐骨神经的破坏完全阻断了BV注射部位热和机械性HA的形成。然而。BV治疗侧后跟的PWTL(降至基线水平)或PWMT(较基线水平下降56%)的减少不受该治疗的影响。然而，破坏坐骨神经和隐神经的CSPA纤维能够阻止BV治疗的整个后爪的热和机械性HA的形成，并阻止未注射BV的后爪的热痛觉过敏。加在一起。我们的结论是：(1)CSPA(C-和A-Delta-)纤维在由S.C.引起的热或机械性痛敏的调节中起着关键作用。(2)CSPA纤维可能在介导福尔马林诱导的PSN中起重要作用，但在BV诱导的伤害性感受过程中起部分作用；(3)除坐骨神经外，隐神经还参与介导BV诱导的PSN以及热和机械痛敏，而不太可能参与福尔马林诱导的伤害性感受。(C)2001年国际疼痛研究协会。爱思唯尔科学公司出版。版权所有。
To investigate the roles of primary afferent fibers in development of the bee venom (BV)-induced persistent spontaneous nociception (PSN) and hyperalgesia (HA), the sciatic nerve or both the sciatic and saphenous nerves of rats were topically treated with capsaicin respectively under pentobarbital anesthesia to destroy the capsaicin-sensitive primary afferent (CSPA) fibers. Effect of the sciatic nerve capsaicin on the formalin-induced PSN was also evaluated. Destruction of the CSPA fibers of the sciatic nerve or both the sciatic and saphenous nerves only produced 34 or 69% inhibition of the mean total number of 1 h BV-induced paw flinches. However, the total number of 1 h formalin-induced paw flinches was inhibited by 90% (85% for phase 1 and 91% for phase 2). In naive rats, destruction of the CSPA fibers of the sciatic nerve caused 237 and 60% increase in paw withdrawal thermal latency (PWTL) to radiant heat in the injection site (paw pad) and at the heel of the treated hind paw compared to the baseline values. However, it was without significant influence upon the: PWTL in the non-treated side or the paw withdrawal mechanical threshold (PWMT) to von Frey filament stimuli in both hind paws. In the BV-treated rats. the CSPA fiber destruction of the sciatic nerve completely blocked development of the heat and mechanical HA in the BV injection site. However. the reduction in either PWTL (drop to baseline level) or PWMT (drop by 56% from the baseline level) at the heel of the BV-treated side was not affected by this treatment. However, destruction of the CSPA fibers of both the sciatic and saphenous nerves was able to block development of both heat and mechanical HA in the whole BV-treated hind paw and heat hyperalgesia in the non-injected hind paw. Taken together. we conclude that: ( 1) the CSPA (C- and A delta-) fibers play a pivotal role in mediation of either the heat or the mechanical hyperalgesia induced by s.c. BV; (2) the CSPA fibers may play a crucial role in mediation of the formalin-induced PSN, but play a partial role in the BV-induced nociceptive process; (3) in addition to the sciatic nerve, the saphenous nerve is also involved in mediation of the BV-induced PSN as well as heat and mechanical hyperalgesia, while it is not likely to be involved in the formalin-induced nociception. (C) 2001 International Association for the Study of Pain. Published by Elsevier Science B.V. All rights reserved.