Metabolite profiling in Arabidopsis.
Metabolite profiling in Arabidopsis.
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拟南芥中的代谢物分析。
DOI：
10.1385/1-59745-003-0:439
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发表时间：
2006
影响因子：
--
通讯作者：
O. Fiehn
中科院分区：
文献类型：
--
作者：
O. Fiehn
代谢物分析是使用色谱和通用检测技术（GC-MS，LC-MS）对化合物或化合物类别的混合物进行多平行相对定量。在这方面，它是经典的单靶方法的延伸，它可以通过其对主要生化事件的分析的更广泛的观点来区分。这种更广泛的分析范围通过在方法开发和特定代谢物准确度降低方面做出妥协而超过了缺点。本章阐述了气相色谱-质谱联用技术（GC-MS）分析极性化合物代谢物的策略。它给出了实验的基本步骤：收获，均质，提取，分馏，浓缩，衍生化，数据采集，原始数据处理和结果数据转换的细节。
Metabolite profiling is the multiparallel relative quantification of a mixture of compounds or compound classes using chromatography and universal detection technologies (GC-MS, LC-MS). In this respect it is an extension of classical single-target methods from which it can be distinguished by its broader view on profiling major biochemical events. This broader scope of analysis outweighs the disadvantages by making compromises in method development and the reduced accuracy for specific metabolites. This chapter exemplifies the strategies in metabolite profiling of polar compounds by gas chromatography-mass spectrometry (GC-MS). It gives experimental details on the basic steps: harvest, homogenization, extraction, fractionation, concentration, derivatization, data acquisition, raw data processing and result data tranformation.