Human growth hormone DNA sequence and mRNA structure: possible alternative splicing.
Human growth hormone DNA sequence and mRNA structure: possible alternative splicing.
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人类生长激素 DNA 序列和 mRNA 结构：可能的选择性剪接。
DOI：
10.1093/nar/9.15.3719
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发表时间：
1981
影响因子：
14.9
通讯作者：
Goodman,HM
中科院分区：
文献类型：
--
作者：
DeNoto,FM;Moore,DD;Goodman,HM
我们测定了人生长激素（hGH）基因的全序列，并确定了hGH mRNA成熟51端的位置。该序列与克隆的hGH cENA序列的比较显示，该基因被四个间插序列中断。SI作图表明其中一个插入序列具有两个不同的3′剪接位点。这些选择性剪接途径产生不同大小的bGH肽，这些肽在正常垂体中发现。将hGH mRNA 5′端附近的序列与人糖蛋白激素α亚基的相似区域进行比较，发现这些无关肽激素之间存在一个意想不到的同源区域。
We have determined the complete sequence of the human growth hormone(hGH) gene and the position of the mature 51 end of the hGH mRNA with in the sequence. Comparison of this sequence with that of a cloned hGH cENA shows that the gene is interrupted by four intervening sequences. SI mapping shows that one of these intervening sequences has two different 3′ splicesites. These alternate splicing pathways generate bGH peptides of differentsizes which are found in normal pituitaries. Comparison of sequences near the 5′ end of the hGH mRNA with a similar region of the α subunit of the human glycoprotein hormones reveals an unexpected region ofhomology between these otherwise unrelated peptide hormones.