Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences
Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences
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DOI：
10.1073/pnas.242603899
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发表时间：
2002-12-24
影响因子：
11.1
通讯作者：
Marra, MA
中科院分区：
文献类型：
--
作者：
Strausberg, RL;Feingold, EA;Marra, MA
美国国立卫生研究院哺乳动物基因收集（MGC）计划是一个多机构的努力，以确定和测序的cDNA克隆含有一个完整的ORF的每个人和小鼠基因。从富集全长cDNA的文库中产生EST，并分析以鉴定候选的全ORF克隆，然后将其测序至高准确度。MGC目前已经测序并验证了一组> 9，000个人类和> 6，000个小鼠基因的完整ORF。另外7，800个人类和3，500个小鼠基因的候选全ORF克隆也已确定。所有MGC序列和克隆都可通过公共数据库和克隆分配网络（参见http://mgc.nci.nih.gov）无限制地获得。
The National Institutes of Health Mammalian Gene Collection (MGC) Program is a multiinstitutional effort to identify and sequence a cDNA clone containing a complete ORF for each human and mouse gene. ESTs were generated from libraries enriched for full-length cDNAs and analyzed to identify candidate full-ORF clones, which then were sequenced to high accuracy. The MGC has currently sequenced and verified the full ORF for a nonredundant set of >9,000 human and >6,000 mouse genes. Candidate full-ORF clones for an additional 7,800 human and 3,500 mouse genes also have been identified. All MGC sequences and clones are available without restriction through public databases and clone distribution networks (see http://mgc.nci.nih.gov).