Discovery of quality-marker ingredients of Panax quinquefolius driven by high-throughput chinmedomics approach
Discovery of quality-marker ingredients of Panax quinquefolius driven by high-throughput chinmedomics approach
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高通量中医医学组学方法发现西洋参质量标志成分
DOI：
10.1016/j.phymed.2019.152928
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发表时间：
2020-08-01
期刊：
影响因子：
7.9
通讯作者：
Wang, Xi-jun
中科院分区：
文献类型：
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作者：
Xiong, Hui;Zhang, Ai-hua;Wang, Xi-jun
背景：中药质量控制一直是中医药研究的热点问题。然而，由于中药成分的复杂性，现行的中药质量标准存在难以保证临床疗效的问题。西洋参是五加科植物西洋参的干燥根，具有多种药理作用和巨大的保健作用，含有人参皂苷等多种活性成分，是一种珍贵的中草药。虽然已有大量研究对西洋参的有效成分进行了研究，但反映西洋参药效的Q标记物尚未得到全面综述。目的：本研究旨在发现西洋参的Q标记物，为阐明西洋参的重要成分提供有力的方法，并有助于进一步发现广泛的质量评价模型，有助于显著提高中药质量标准。方法：利用小鼠一般情况、生化指标测定、尿代谢谱和血清代谢谱进行模型复制和疗效评价。采用中药血清药物化学联用技术，对西洋参的体外和体内化学成分进行了深入研究。结果：对41种血、尿标记代谢物与14种血清成分的相关性分析表明，24-甲基-7-胆固酮-3-β-醇、酸枣仁皂苷II、白桦醇、人参皂苷Rd、肉桂醇、人参皂苷F11与复方枣饭丸的疗效高度相关，伪人参皂苷F11、人参皂苷Rd与西洋参的疗效高度相关。这6种被吸收的血液化合物可作为化合物的潜在Q标记，其中三七皂苷F11和人参皂苷Rd可作为西洋参的潜在Q标记。结论：本研究证明金氏化学是一种发现中药Q标记的有效、全面、全新的方法，可能是制定中药质量标准的更合理的选择。
Background: Quality control of traditional Chinese medicine (TCM) has always been a hot issue to TCM. However, due to the complexity of TCM ingredients, the current quality standards of TCM have problems that are difficult to guarantee clinical efficacy. American ginseng, the dried roots of Pawajc quinquefolium L. (Araliaceae), is a valuable herbal medicine due to various pharmacological effects and huge health benefit, which are associated with numerous active ingredients such as ginsenosides. Although a large number of studies have investigated the active ingredients of American ginseng, Q-markers reflecting comprehensive review on its efficacies has yet been unrevealed.Purpose: The study aims to discover the Q-markers of Panax quinquefolius (American ginseng), provides a powerful method to clarify the significant ingredents of TCM and help further discovering extensive quality evaluation model,contributing to a significant improvement of TCM quality standard.Methods: Mice general status, biochemical indexes assay, urine metabolic profile, and serum metabolic profile were utilized for model replication and efficacy evaluation. The in vitro and in vivo constituents of American ginseng using ultra-high performance liquid chromatography coupled with mass spectrometry (UPLC-MS) with Serum Pharmacochemistry of TCM were in-depth investigated. Q-markers that were associated with core markers of therapeutic effects were excavated by a plotting of correlation between marker metabolites and serum constituents (PCMS) approach.Results: Correlation analysis of 41 blood and urine labeled metabolites with 14 serum components showed that 24-methyl-7-cholesten-3 beta-ol, zizybeoside II, betulin, ginsenoside Rd, cinnamyl alcohol, pseudoginsenoside F11 is highly correlated with the therapeutic effects of Compound Zaofan Pill (CZP), while pseudoginsenoside F11 and ginsenoside Rd are highly correlated with the therapeutic effects of American ginseng. The six absorbed blood compounds can be considered as potential Q-markers for compound, of which two compounds, such as pseudoginsenoside F11 and ginsenoside Rd, can be considered as potential Q-markers for American ginseng.Conclusion: The study has demonstrated that the Chinmedomics is an effective, comprehensive and fire-new method for discovering the Q-markers of TCM, and it may be more reasonable choices to establish quality standards of TCM.