Occurrence of tetracycline resistance genes tet(M) and tet(S) in bacteria from marine aquaculture sites
Occurrence of tetracycline resistance genes tet(M) and tet(S) in bacteria from marine aquaculture sites
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DOI：
10.1016/j.femsle.2004.06.026
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发表时间：
2004-08-01
影响因子：
2.1
通讯作者：
Suzuki, S
中科院分区：
文献类型：
--
作者：
Kim, SR;Nonaka, L;Suzuki, S
在日本和韩国沿海水产养殖场的鱼类和海水中分离的151株四环素耐药细菌中，检测了编码核糖体保护蛋白的四环素耐药基因的发生。在34株日本和韩国分离株中检测到泰特（M）基因，其中包括弧菌属，Garvieae乳球菌、美丽发光杆菌亚种。杀鱼动物和未鉴定的革兰氏阳性菌。这些细菌分离株中的大多数显示出高水平的耐药性，最小抑制浓度（MIC）等于或大于250 μ g/ml的土霉素，只有4个分离株的MIC小于31.3 μ g/ml。泰特（M）阳性弧菌分离株的16 S rDNA RFLP分型表明，这些是特定于特定位置的相同弧菌类型的克隆群体。然而，在日本和韩国，一种弧菌克隆（弧菌III型）广泛传播，在不同的采样年份、不同的地点和不同的鱼类中被检测到。在L.来自日本黄尾鱼的garvieae和来自韩国海水的弧菌属。这是第一次报告泰特（S）发生在革兰氏阴性兼性厌氧菌。这些结果表明，泰特（M）和泰特（S）基因存在于鱼类肠道和海水细菌在养殖场，可能是一个重要的水库四环素耐药基因在海洋环境中。(C)2004年，欧洲微生物学会联合会。Elsevier B. V.出版，保留所有权利。
Occurrence of tetracycline resistance genes encoding ribosomal protection proteins was examined in 151 tetracycline-resistant bacterial isolates from fish and seawater at coastal aquaculture sites in Japan and Korea. The tet(M) gene was detected in 34 Japanese and Korean isolates, which included Vibrio sp., Lactococcus garvieae, Photobacterium damsela subsp. piscicida, and unidentified Gram-positive bacteria. The majority of these bacterial isolates displayed high-level resistance with a minimum inhibitory concentrations (MICs) equal to or greater than 250 mug/ml of oxytetracycline and only four isolates had MICs less than 31.3 mug/ml. 16S rDNA RFLP typing of tet(M)-positive Vibrio isolates suggests that these are clonal populations of the same phylotype specific to a particular location. One Vibrio clone (phylotype III), however, is widely disseminated, being detected during different sampling years, at different locations, and in different fish species in both Japan and Korea. The tet(S) gene was detected in L. garvieae from yellowtail in Japan and in Vibrio sp. from seawater in Korea. This is the first report of tet(S) occurrence in Gram-negative facultative anaerobes. These results suggest that tet(M) and tet(S) genes are present in fish intestinal and seawater bacteria at aquaculture sites and could be an important reservoir of tetracycline resistance genes in the marine environment. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.