CA9 transcriptional expression determines prognosis and tumour grade in tongue squamous cell carcinoma patients
CA9 transcriptional expression determines prognosis and tumour grade in tongue squamous cell carcinoma patients
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CA9转录表达决定舌鳞状细胞癌患者的预后和肿瘤分级
DOI：
10.1111/jcmm.15252
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发表时间：
2020-05-01
影响因子：
5.3
通讯作者：
Liang, Yujie
中科院分区：
文献类型：
--
作者：
Guan, Chenyu;Ouyang, Daiqiao;Liang, Yujie
CA 9是碳酸酐酶家族的成员，常在缺氧条件下的癌细胞中表达。然而，CA 9在舌鳞状细胞癌（TSCC）发病机制中的作用仍不清楚。使用TCGA数据库分析CA 9表达，并使用Kaplan-Meier、LASSO和考克斯回归分析进行其对生存的影响。采用CIBERSORT和ESTIMATE分析CA 9与免疫浸润的相关性。并通过GSEA、GO和WGCNA分析CA 9表达与下游分子调控途径的关系。TSCC中CA 9的表达与临床预后和肿瘤分级相关。此外，CA 9表达可能有助于调节癌细胞分化并介导肿瘤相关基因和信号传导途径，包括凋亡、缺氧、G2 M检查点、PI 3 K/AKR/mTOR信号传导和TGF-β信号传导途径。而CA 9高表达组和低表达组的滤泡辅助性T细胞、调节性T细胞、免疫和间质评分均无显著性差异。这些结果表明，CA 9在TSCC预后和肿瘤分级中起着关键作用。CA 9表达与细胞分化、多种癌基因和癌症相关通路的调节显著相关。
CA9 is a member of the carbonic anhydrases' family, that is often expressed in cancer cells under hypoxic condition. However, the role of CA9 in the molecular mechanisms of tongue squamous cell carcinoma (TSCC) pathogenesis remains unclear. CA9 expression was analysed using the TCGA database, and its influence on survival was performed using Kaplan-Meier, LASSO and COX regression analyses. The correlation between CA9 and immune infiltration was investigated by CIBERSORT and ESTIMATE. Moreover, the relationship between CA9 expression and downstream molecular regulation pathways was analysed by GSEA, GO and WGCNA. CA9 expression correlated with clinical prognosis and tumour grade in TSCC. Moreover, CA9 expression potentially contributes to the regulation of cancer cell differentiation and mediates tumour-associated genes and signalling pathways, including apoptosis, hypoxia, G2M checkpoint, PI3K/AKR/mTOR signalling and TGF-beta signalling pathways. However, the follicular helper T cells, regulatory T cells, immune and stromal scores showed no significance between high and low CA9 expression groups. These findings suggested that CA9 plays a critical role of TSCC prognosis and tumour grade. CA9 expression significantly correlated with the regulation of cell differentiation, various oncogenes and cancer-associated pathways.