Detection of the Na(+)-K(+)-ATPase alpha 3-isoform in multinucleated macrophages.
Detection of the Na(+)-K(+)-ATPase alpha 3-isoform in multinucleated macrophages.
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检测多核巨噬细胞中的 Na( )-K( )-ATPase α 3-亚型。
DOI：
10.1152/ajprenal.1991.260.5.f704
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Gilmore-Hebert,M
中科院分区：
文献类型：
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作者：
Vignery,A;Wang,F;Qian,HY;BenzJr,EJ;Gilmore-Hebert,M
我们先前报道，多核巨噬细胞表达高浓度的Na(+)-K(+)-ATPase，这些酶集中在其质膜的非粘附区(A.Vignery，T.Niven-FairChild，D.H.Ingbar和M.Caplan)。J.组织化学。细胞化学。37：1265-1271,1989)。我们还发现，在细胞培养和多核形成过程中，新合成的α-亚基的增加。我们现在提出的证据表明，巨噬细胞体外多核伴随着Na(+)-K(+)-ATPaseα亚单位mRNA的增加。最有趣的是通过原位杂交、Northern和Western印迹分析在这些细胞中检测到大量的α1和α3亚型mRNA和多肽。这些Na(+)-K(+)-ATPase表达的质和量的变化表明，巨噬细胞的多核伴随着基因表达的协调调节，多核赋予巨噬细胞特定的功能。多核巨噬细胞提供了一个新的模型系统，不仅可以研究α3-亚型的特殊功能(S)，而且还可以研究Na(+)-K(+)-ATPase在巨细胞和破骨细胞中的作用。
We previously reported that multinucleated macrophages express a high concentration of Na(+)-K(+)-ATPases that are concentrated on the nonadherent domain of their plasma membrane (A. Vignery, T. Niven-Fairchild, D. H. Ingbar, and M. Caplan. J. Histochem. Cytochem. 37: 1265-1271, 1989). We also showed that an increase in newly synthesized alpha-subunit occurred during cell culture and multinucleation. We now present evidence that macrophage multinucleation in vitro is accompanied by an increased accumulation of Na(+)-K(+)-ATPase alpha-subunit mRNA. Most interesting is the detection of significant amount of both alpha 1- and alpha 3-isoform mRNA and peptide in these cells by in situ hybridization, Northern and Western blot analyses. These qualitative and quantitative variations in Na(+)-K(+)-ATPase expression suggest that macrophage multinucleation is accompanied by a coordinated regulation of gene expression and that multinucleation confers a specific function to macrophages. Multinucleated macrophages offer a novel model system to investigate not only the specific function(s) of the alpha 3-isoform but also the role of the Na(+)-K(+)-ATPase in giant cells and osteoclasts.