Autologous patient serum for the culture of keratinocyte transplants reduces risk of transmittable disease
Autologous patient serum for the culture of keratinocyte transplants reduces risk of transmittable disease
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用于角质形成细胞移植培养的自体患者血清可降低传染性疾病的风险
DOI：
10.1016/s0140-6736(98)05950-9
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
S. Hess
中科院分区：
文献类型：
--
作者：
H. Smola;M. Reinke;P. Shephard;T. Krieg;S. Hess
自体角质形成细胞移植用于治疗愈合不良的伤口，如慢性下肢静脉溃疡。1从患者的小皮肤样品开始，分离能够增殖的基底角质形成细胞，然后通常通过Rheinwald和绿色的方法在组织培养中扩增。2与生长抑制的3T3饲养细胞和含有胎牛血清（FCS）的复合培养基共培养对角质形成细胞的持续增殖是必需的。3一旦获得足够数量的角质形成细胞，就可以制备移植物制剂。来自非自体血清的产品，特别是FCS，具有传播病毒和朊病毒等传染性病原体的风险。然而，自体或同种异体角质形成细胞移植的益处似乎超过了感染的风险，因为监管机构对此问题几乎没有引起关注。我们用自体患者血清替代FCS，尽管患者在治疗前和治疗后都没有发现角质形成细胞生长的任何差异，
Autologous keratinocyte grafting is used for treating poorly healing wounds such as chronic venous leg ulcers. 1 Starting with a small skin sample from the patient, basal keratinocytes capable of proliferating are isolated and then expanded in tissue culture usually by the method of Rheinwald and Green. 2 Co-culture with growth-inhibited 3T3 feeder cells and complex medium containing fetal calf serum (FCS) enriched with growth supplements is essential for sustained keratinocyte proliferation. 3 Once a sufficient number of keratinocytes has been obtained, graft preparations can be prepared. Products derived from non-autologous serum, and FCS in particular, carry the risk of transmitting infectious agents such as viruses and prions. The benefits, however, of autologous or allogenic keratinocyte grafting seem to outweigh the risks of infection as regulatory authorities have raised little concern over this issue. We substituted autologous patient serum to FCS and noted little if any difference in keratinocyte growth despite the patients being
在自发永生的非整倍性人角质形成细胞系中的正常角质化。
DOI：
10.1083/jcb.106.3.761
发表时间：
1988-03
期刊：
The Journal of cell biology
影响因子：
--
作者：
Boukamp P;Petrussevska RT;Breitkreutz D;Hornung J;Markham A;Fusenig NE
通讯作者：
Fusenig NE