Collision-induced dissociation of mobility-separated ions using an orifice-skimmer cone at the back of a drift tube
Collision-induced dissociation of mobility-separated ions using an orifice-skimmer cone at the back of a drift tube
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DOI：
10.1021/ac010295e
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发表时间：
2001-08-01
影响因子：
7.4
通讯作者：
Clemmer, DE
中科院分区：
文献类型：
--
作者：
Lee, YJ;Hoaglund-Hyzer, CS;Clemmer, DE
本文提出了一种在漂移管出口处引入差分泵吸的分离锥（OSC）区的离子迁移率质谱技术。OSC区域在设计上类似于电喷雾电离源中所使用的那些，并且提供了离子传输的改进（与先前的设计相比，通过类似于5 -10的因子）和诱导迁移率分离的离子的碎裂的能力。在分离锥处解离之前分离离子允许检查碎片离子的来源。在这里，我们描述了实验设计，并通过检查碎片离子，共同的多个电荷状态和不同的气相离子构象的电喷雾血管紧张素II和[Sar（1），瓦尔（5），Ala（8）]血管紧张素II肽的方法证明。
An ion mobility-mass spectrometry technique that incorporates a differentially pumped orifice-skimmer cone (OSC) region at the exit of the drift tube has been developed. The OSC region is similar in design to those used in electrospray ionization sources and offers improvements in ion transmission (by factors of similar to5-10 compared with previous designs) and the ability to induce fragmentation of mobility-separated ions. The separation of ions prior to dissociation at the skimmer cone allows the origin of fragment ions to be examined. Here, we describe the experimental design and demonstrate the approach by examining fragment ions that are common to multiple charge states and different gas-phase ion conformations of electrosprayed angiotensin II and [Sar(1), Val(5), Ala(8)] angiotensin II peptides.