Post-translational modification accounts for the presence of varied forms of nerve growth factor in Australian elapid snake venoms
Post-translational modification accounts for the presence of varied forms of nerve growth factor in Australian elapid snake venoms
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DOI：
10.1002/pmic.200600263
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发表时间：
2006-12-01
期刊：
影响因子：
3.4
通讯作者：
Lavin, Martin F.
中科院分区：
文献类型：
--
作者：
Earl, Stephen T. H.;Birrell, Geoff W.;Lavin, Martin F.
澳大利亚眼镜蛇是世界上最毒的蛇之一，但与亚洲和美洲蛇相比，对整体毒液成分的了解要少得多。本研究采用cDNA克隆和双向电泳结合质谱的方法，对澳洲眼镜蛇蛇毒中的神经生长因子（NGF）进行了鉴定，并证明了其神经突生长活性。使用2-DE鉴定了在不同蛇内和不同蛇之间具有相当大的分子大小变化的NGF蛋白。大小的变化可以至少部分地通过Winked糖基化来解释。这些修饰可能改变了蛇NGF的稳定性、活性和其他特性。进一步表征描绘了单个NGF同种型的功能。
The Australian elapid snakes are amongst the most venomous snakes in the world, but much less is known about the overall venom composition in comparison to Asian and American snakes. We have used a combined approach of cDNA cloning and 2-DE with MS to identify nerve growth factor (NGF) in venoms of the Australian elapid snakes and demonstrate its neurite outgrowth activity While a single 730 nucleotide ORF, coding for a 243 amino acid precursor protein was detected in all snakes, use of 2-DE identified NGF proteins with considerable variation in molecular size within and between the different snakes. The variation in size can be explained at least in part by Winked glycosylation. it is possible that these modifications alter the stability, is necessary to activity and other characteristics of the snake NGFs. Further characterisation delineate the function of the individual NGF isoforms.