Influence of different crosslinking treatments on the physical properties of collagen membranes
Influence of different crosslinking treatments on the physical properties of collagen membranes
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DOI：
10.1016/s0142-9612(02)00412-x
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发表时间：
2003-02-01
期刊：
影响因子：
14
通讯作者：
Rajaram, A
中科院分区：
文献类型：
--
作者：
Charulatha, V;Rajaram, A
胶原基生物材料的物理性能受交联方法和交联程度的影响。在这项研究中，各种交联处理对重构胶原蛋白膜的物理性质的影响进行了评估。五种交联剂，采用GTA、DMS、DTBP、DMS + GTA及酰基叠氮法稳定胶原基质。测定交联密度、溶胀比、热机械性能、应力-应变特性和抗胶原酶消化性，以评价交联基质的物理性能。GTA处理诱导了最大数量的交联（13），而DMS处理诱导了最小数量的交联（7）。在两种二亚胺酯（DMS和DTBP）中，由于DTBP交联中存在二硫键，DTBP是更有效的交联剂。DTBP和DMS交联胶原的T-s分别为80 ℃和70 ℃，它们的HIT值分别为5.4和2.85 MN/m（2）。低浓度的GTA（0.01%）将已经交联的基质（DMS处理的基质）的交联密度从7增加到12。对于酰基叠氮化物处理的基质观察到最低的断裂能（0.61 MJ/m（3）），而对于GTA处理的基质观察到最高的断裂能（1.97 MJ/m（3））。GTA处理的基体拉伸强度最大（12.4MPa），而酰基叠氮处理的基体拉伸强度最小（7.2MPa）。GTA、DTBP和酰基叠氮化物处理的基质对胶原酶降解的抗性相同，5小时后溶解度约为6%，而DMS处理的基质最不稳定，相同时间段后溶解度为52.4%。胶原蛋白上氨基酸侧链残基的空间取向在确定胶原蛋白基质的交联密度和随后的物理性质中起重要作用。(C)2002爱思唯尔科技有限公司版权所有。
The physical properties of collagen-based biomaterials are profoundly influenced by the method and extent crosslinking. In this study, the influence of various crosslinking treatments on the physical properties of reconstituted collagen membranes was assessed. Five crosslinking agents viz., GTA, DMS, DTBP, a combination of DMS and GTA and acyl azide method were used to stabilize collagen matrices. Crosslinking density, swelling ratio, thermo-mechanical properties, stress-strain characteristics and resistance to collagenase digestion were determined to evaluate the physical properties of crosslinked matrices. GTA treatment induced the maximum number of crosslinks (13) while DMS treatment induced the minimum (7). Of the two diimidoesters (DMS and DTBP), DTBP was a more effective crosslinking agent due to the presence of disulphide bonds in the DTBP crosslinks. T-s for DTBP and DMS crosslinked collagen were 80degreesC and 70degreesC, and their HIT values were 5.4 and 2.85 MN/m(2), respectively. Low concentration of GTA (0.01%) increased the crosslinking density of an already crosslinked matrix (DMS treated matrix) from 7 to 12. Lowest fracture energy was observed for the acyl azide treated matrix (0.61 MJ/m(3)) while the highest was observed for the GTA treated matrix (1.97 MJ/m(3)). The tensile strength of GTA treated matrix was maximum (12.4 MPa) and that of acyl azide treated matrix was minimum (7.2 MPa). GTA, DTBP and acyl azide treated matrices were equally resistant to collagenase degradation with approximate to6% solubilization after 5 h while the DMS treated was least stable with 52.4% solubilization after the same time period. The spatial orientation of amino acid side chain residues on collagen plays an important role in determining the crosslinking density and consequent physical properties of the collagen matrix. (C) 2002 Elsevier Science Ltd. All rights reserved.