Conserved nucleosome positioning defines replication origins
Conserved nucleosome positioning defines replication origins
复制标题
DOI：
10.1101/gad.1913210
复制
发表时间：
2010-04-15
影响因子：
10.5
通讯作者：
MacAlpine, David M.
中科院分区：
文献类型：
--
作者：
Eaton, Matthew L.;Galani, Kyriaki;MacAlpine, David M.
原点识别综合体（ORC）指定复制原点位置。酿酒酵母ORC识别ARS（自主复制序列）共有序列（ACS），但只有潜在基因组位点的一部分是结合的，这表明其他染色体特征会影响ORC结合。使用高通量测序对映射兽人结合和核小体定位，我们表明酵母的起源的特征是ACS侧面的核小体的不对称模式。原点序列足以维持无核小体的起源。但是，兽人的精确定位是核心小体侧面的精确定位所必需的。这些发现将局部核小体确定为起源选择和功能的重要决定因素。
The origin recognition complex (ORC) specifies replication origin location. The Saccharomyces cerevisiae ORC recognizes the ARS (autonomously replicating sequence) consensus sequence (ACS), but only a subset of potential genomic sites are bound, suggesting other chromosomal features influence ORC binding. Using high-throughput sequencing to map ORC binding and nucleosome positioning, we show that yeast origins are characterized by an asymmetric pattern of positioned nucleosomes flanking the ACS. The origin sequences are sufficient to maintain a nucleosome-free origin; however, ORC is required for the precise positioning of nucleosomes flanking the origin. These findings identify local nucleosomes as an important determinant for origin selection and function.