The interaction of white spot syndrome virus envelope protein VP28 with shrimp Hsc70 is specific and ATP-dependent.
The interaction of white spot syndrome virus envelope protein VP28 with shrimp Hsc70 is specific and ATP-dependent.
复制标题
DOI：
10.1016/j.fsi.2009.01.001
复制
发表时间：
2009-03
影响因子：
4.7
通讯作者：
Hua Xu;F. Yan;Xiaobei Deng;Jiancheng Wang;Tingting Zou;Xin Ma;Xu Zhang;Y. Qi
中科院分区：
文献类型：
--
作者：
Hua Xu;F. Yan;Xiaobei Deng;Jiancheng Wang;Tingting Zou;Xin Ma;Xu Zhang;Y. Qi
白色斑点综合征病毒（White Spot Syndrome Virus，WSSV）是对虾和其他甲壳类动物最具破坏性的病原体之一。在先前的研究中，我们证明了WSSV的主要包膜蛋白VP 28参与了对虾细胞的附着和渗透。本研究表明，对虾热休克同源蛋白70（Hsc 70）是病毒感染过程中VP 28的结合伴侣。Hsc 70在WSSV感染早期表达增强，12 h达高峰，后期表达急剧下降。在对虾血细胞中，VP 28和Hsc 70蛋白定位于细胞质中，并且它们的关联是特异性的、ATP依赖性的和Hsc 70浓度依赖性的。此外，VP 28-Hsc 70的直接结合需要Hsc 70的ATP酶结构域和肽结合结构域。这些结果将有助于阐明VP 28蛋白在病毒感染过程中的作用。
White spot syndrome virus (WSSV) is one of the most devastating pathogens of shrimps and other crustaceans. In a previous study, we demonstrated that the major envelope protein VP28 of WSSV was involved in the attachment and penetration into shrimp cells. Here we showed that heat-shock cognate protein 70 (Hsc70) of shrimp was a binding partner of VP28 during virus infection. Hsc70 expression was enhanced by WSSV infection at the early stage and peaked at 12h post-infection and decreased drastically at the late stage. In shrimp haemocytes, both VP28 and Hsc70 proteins localized in the cytoplasm and their association was specific, ATP-dependent and Hsc70 concentration dependent. Moreover, direct VP28–Hsc70 association required both ATPase domain and peptide binding domain of Hsc70. The data obtained will help elucidate the role of VP28 protein in the process of virus infection.